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024 7 _a10.1007/978-1-0716-3946-7
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245 1 0 _aCancer Cytogenetics and Cytogenomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Jing Christine Ye, Henry H. Heng.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXI, 397 p. 79 illus., 63 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2825
505 0 _aThe New Era of Cancer Cytogenetics and Cytogenomics -- Methods of Detection and Mechanisms of Origin of Complex Structural Genome Variations -- A Paradoxical Role for Somatic Chromosomal Mosaicism and Chromosome Instability in Cancer: Theoretical and Technological Aspects -- The Importance of Monitoring Non-Clonal Chromosome Aberrations (NCCAs) in Cancer Research -- Optical Genome Mapping: A Case Study for Machine-Based Platforms in Cytogenetics and Cytogenomics -- Cell Culture and Slide Preparation for Cytogenetic Studies of Hematological Neoplasms -- Chromosome Bandings and Recognition -- Cancer Sample Analysis Utilizing Single-Nucleotide Polymorphism Array and Array Comparative Genomic Hybridization -- Multitarget Fluorescence In Situ Hybridization Diagnostic Applications in Tumors -- High-Resolution FISH Analysis Using DNA Fibers Generated by Molecular Combing -- FISH-Banding for Characterization of Simple and Complex Chromosomal Rearrangements in Cancer -- Profiling Chromosome Topological Features by Super-Resolution 3D Structured Illumination Microscopy -- Quantitative FISHing: Implications for Chromosomal Analysis -- Characterizing Chemotherapy/Radiotherapy-Induced Genome Chaos in Hodgkin’s Lymphoma Patients Using M-FISH -- Tracking Karyotype Changes in Treatment-Induced Drug-Resistant Evolution -- Generation of Polyploid Giant Cancer Cells (PGCCs) from Hey Ovarian Cancer Cells and Analysis of Embryonic Properties -- Single-Cell MTT: A Simple and Sensitive Assay for Determining the Viability and Metabolic Activity of Polyploid Giant Cancer Cells (PGCCs) -- Evaluating Chromosome Instability and Genotoxicity Through Single-Cell Quantitative Imaging Microscopy -- Studying the Dynamics of Tunneling Tubes and Cellular Spheres -- Profiling Numerical and Structural Chromosomal Instability in Different Cancer Types -- The Digital World of Cytogenetic and Cytogenomic Web Resources.
520 _aThis detailed volume explores the frontiers of this new era in cancer cytogenetics and cytogenomics, focusing on establishing a karyotype as an information-based genomic framework, as well as presenting technological platforms for collecting and analyzing data at the genome level. It begins with several conceptual chapters that introduce ideas such as the Genome Architecture Theory, forcefully emphasizing the importance of cytogenomics in the post-genomics era. The book then proceeds with protocols covering both basic and advanced cytogenetic and cytogenomic methods, as well as diverse experiments beyond traditional cytogenetic platforms, and bioinformatics techniques and resources. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cancer Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal guide to the unique power of this area of research in spatial biology and system-integrated genomics.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCancer
_xGenetic aspects.
650 0 _aCytology
_xTechnique.
650 1 4 _aCancer Genetics and Genomics.
650 2 4 _aCytological Techniques.
700 1 _aYe, Jing Christine.
_eeditor.
_0(orcid)0000-0002-6163-3317
_1https://orcid.org/0000-0002-6163-3317
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHeng, Henry H.
_eeditor.
_0(orcid)0000-0003-1475-9547
_1https://orcid.org/0000-0003-1475-9547
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071639450
776 0 8 _iPrinted edition:
_z9781071639474
776 0 8 _iPrinted edition:
_z9781071639481
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2825
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3946-7
912 _aZDB-2-PRO
999 _c8629
_d8629