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020 _a9781071636213
_9978-1-0716-3621-3
024 7 _a10.1007/978-1-0716-3621-3
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aSingle Cell Analysis
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Miodrag Gužvić.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXIII, 260 p. 54 illus., 45 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2752
505 0 _aSingle Cell Isolation from Surgically Resected Tissue via Mechanical Dissociation using TissueGrinder,- Circulating Tumor Cell Enrichment and Single Cell Isolation Combining the CellSearch® and DEPArray™ Systems -- Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer Patients -- Single Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasis Initiating Cells -- Isolation of Single Circulating Tumor Cells using VyCAP Puncher System -- Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell -- Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients -- Establishing Single Cell Clones from In Vitro Cultured Circulating Tumor Cells -- Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Proteins and Transcripts in Stress Granules -- Highly Multiplexed and Simultaneous Characterization of Protein and RNA in Single Cells by Flow or Mass Cytometry Platforms using Proximity Ligation Assay for RNA -- Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells -- Single Cell MicroRNA Sequencing Library Preparation -- Immunoblot Analysis from Single Cells using Milo™ Single-Cell Western Platform -- Imaging of Sub-Cellular Distribution of Platinum in Single Cells using Laser Ablation Inductively Coupled Plasma Mass Spectrometry -- Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression -- Single-Nucleus ATAC-seq for Mapping Chromatin Accessibility in Individual Cells of Murine Hearts.
520 _aThis volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy such as ICP-MS; and sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Single Cell Analysis: Methods and Protocols is a valuable tool for any researcher interested in learning more about this important and developing field. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCytology.
650 0 _aMaterials
_xMicroscopy.
650 1 4 _aCell Biology.
650 2 4 _aMicroscopy.
700 1 _aGužvić, Miodrag.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071636206
776 0 8 _iPrinted edition:
_z9781071636220
776 0 8 _iPrinted edition:
_z9781071636237
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2752
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3621-3
912 _aZDB-2-PRO
999 _c8617
_d8617