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020 _a9781071629789
_9978-1-0716-2978-9
024 7 _a10.1007/978-1-0716-2978-9
_2doi
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072 7 _aSCI013040
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082 0 4 _a572.6
_223
245 1 0 _aSerum/Plasma Proteomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by David W. Greening, Richard J. Simpson.
250 _a3rd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXVIII, 596 p. 60 illus., 50 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2628
505 0 _aProtocols for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion -- Collection of plasma samples in areas with limited healthcare access -- Blood Collection Processing & Handling for Plasma and Serum Proteomics -- Preparation of cryoprecipitate and cryo-depleted plasma for proteomic research analysis -- High throughput proteome profiling of plasma and native plasma complexes using native chromatography -- High-throughput and in-depth proteomic profiling of 5 µL plasma and serum using TMTpro 16plex -- In depth data-independent acquisition proteomics of human plasma identifies low abundant proteins -- In-depth Blood Proteome Profiling by Extensive Fractionation and multiplexed quantitative mass spectrometry -- Early cancer biomarker discovery using DIA-MS proteomic analysis of EVs from peripheral blood -- Strategies to enrich, identify and characterize glycoproteome in blood plasma using Liquid Chromatography High Resolution Mass Spectrometry -- Proteome analysis of whole blood collected by volumetric absorptive microsampling -- Proteomic applications and considerations: from research to patient care -- Immunoaffinity mass spectrometry diagnostic tests for multi-biomarker assays -- Secretome profile of leukocyte-platelet rich fibrin (L-PRF) membranes -- Quantification of proteins in blood by absorptive microtiter plate-based affinity purification coupled to liquid chromatography-mass spectrometry -- Glycomics-assisted Glycoproteomics Enables Deep and Unbiased N-Glycoproteome Profiling of Complex Biological Specimens. -The Small Protein Enrichment Assay (SPEA) for analysis of low abundance peptide hormones in plasma -- In-depth proteomic analysis of blood circulating small extracellular vesicles -- Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-based Proteomic Identification -- Analysis of ExtracellularVesicle and Contaminant Markers in Blood Derivatives Using Multiple Reaction Monitoring -- Generation of red blood cell nanovesicles as a delivery tool -- Progress in targeted mass spectrometry (parallel accumulation-serial fragmentation) and its application in plasma/serum proteomics -- Offline peptide fractionation and parallel reaction monitoring MS for the quantitation of low-abundance plasma proteins -- Profiling Serum Intact N-Glycopeptides Using Data-Independent Acquisition Mass Spectrometry -- Semi-automated lectin magnetic bead array (LeMBA) for translational serum glycoprotein biomarker discovery and validation -- Accessing antibody-reactivities in serum or plasma to (auto-)antigens using multiplexed bead-based protein immunoassays -- Absolute quantitative targeted proteomics assays for plasma proteins -- Rapid and quantitative enrichment of peptides from plasma for mass spectrometric analysis -- Comprehensive targeted lipidomic profilingfor research and clinical applications -- Multiplexed bead-based peptide immunoassays for the detection of antibody reactivities -- Array-based multiplex and high-throughput serology assays -- Bioinformatic tools and knowledgebases to assist generating targeted assays for plasma proteomics -- Use of Longitudinal Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early Detection.
520 _aThis third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aProteins.
650 0 _aBioinformatics.
650 0 _aMass spectroscopy.
650 1 4 _aProteins.
650 2 4 _aBioinformatics.
650 2 4 _aMass Spectrometry.
700 1 _aGreening, David W.
_eeditor.
_0(orcid)0000-0001-7516-485X
_1https://orcid.org/0000-0001-7516-485X
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aSimpson, Richard J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071629772
776 0 8 _iPrinted edition:
_z9781071629796
776 0 8 _iPrinted edition:
_z9781071629802
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2628
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2978-9
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