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| 001 | 978-1-0716-2671-9 | ||
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_a10.1007/978-1-0716-2671-9 _2doi |
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_aChromaffin Cells _h[electronic resource] : _bMethods and Protocols / _cedited by Ricardo Borges. |
| 250 | _a1st ed. 2023. | ||
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_aNew York, NY : _bSpringer US : _bImprint: Humana, _c2023. |
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_aXIV, 373 p. 89 illus., 68 illus. in color. _bonline resource. |
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_acomputer _bc _2rdamedia |
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_aonline resource _bcr _2rdacarrier |
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_bTable of contents navigation _2onix |
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_bSingle logical reading order _2onix |
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_bShort alternative textual descriptions _2onix |
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_bUse of color is not sole means of conveying information _2onix |
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_atext file _bPDF _2rda |
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_aMethods in Molecular Biology, _x1940-6029 ; _v2565 |
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| 505 | 0 | _aRat pheochromocytoma PC12 cells in culture -- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation -- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells -- Transmission electron microscopy: a method for studying the adrenochromaffin cells -- Immunogold for protein location in chromaffin cells -- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy -- Online detection of catecholamine release from the perfused rat adrenal gland -- On-line recording of perifused chromaffin cells -- Recording of chromaffin cell electrical activity in situ in acute adrenal slices -- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats -- Measurements of calcium in chromaffin cell organelles using targeted aequorins -- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements -- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell -- Quantal release analysis of electrochemically active molecules using single-cell amperometry -- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs -- Vesicle collision protocols for the study of quantum size and exocytotic fraction released -- Patch Amperometry and Intracellular Patch Electrochemistry -- Artificial cells for dissecting exocytosis -- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells -- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells -- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging -- Determinationof catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein -- Quantification of chromogranin A and its fragments in biological fluids -- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds. | |
| 520 | _aThis volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. . | ||
| 532 | 8 | _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. | |
| 532 | 8 | _aNo reading system accessibility options actively disabled | |
| 532 | 8 | _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com | |
| 650 | 0 | _aCytology. | |
| 650 | 0 | _aPharmacology. | |
| 650 | 1 | 4 | _aCell Biology. |
| 650 | 2 | 4 | _aPharmacology. |
| 700 | 1 |
_aBorges, Ricardo. _eeditor. _0(orcid)0000-0003-3413-4392 _1https://orcid.org/0000-0003-3413-4392 _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071626702 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071626726 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071626733 |
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_aMethods in Molecular Biology, _x1940-6029 ; _v2565 |
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| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-1-0716-2671-9 |
| 912 | _aZDB-2-PRO | ||
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