000 05132nam a22005175i 4500
001 978-1-59259-642-3
003 DE-He213
005 20260610083723.0
007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592596423
_9978-1-59259-642-3
024 7 _a10.1007/978-1-59259-642-3
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aMolecular Biomethods Handbook
_h[electronic resource] /
_cedited by Ralph Rapley, John M. Walker.
250 _a1st ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aXIII, 725 p. 360 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Protocols Handbooks,
_x1949-2456
505 0 _aExtraction of Total RNA from Tissues and Cultured Cells -- Isolation of Total Cellular DNA from Tissues and Cultured Cells -- Gel Electrophoresis of DNA -- Sl Nuclease Analysis of RNA -- Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA) -- Gene Probes -- Nonradioactive Labeling of DNA -- Southern Blot Analysis -- Northern Blot Analysis -- DNA Sequencing -- Autoradiography and Fluorography -- Mobility Shift Assays -- cDNA Libraries -- Genomic DNA Libraries -- ? as a Cloning Vector -- Plasmid-Derived Cloning Vectors -- M13 and Phagemid-Based Cloning Vectors -- Retroviral Vectors From Laboratory Tools to Molecular Medicines -- Baculovirus Vectors -- Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes -- Plant Transformation -- Restriction Fragment-Length Polymorphisms -- Genome Mapping -- Yeast Artificial Chromosomes -- Polymerase Chain Reaction -- In Vitro Transcriptions -- In Vitro Translation -- Site-Directed Mutagenesis -- Site-Directed Mutagenesis -- DNA Profiling -- Radiolabeling of Peptides and Proteins -- Protein Electrophoresis -- Free Zone Capillary Electrophoresis -- Protein Blotting -- Ion-Exchange Chromatography -- Size-Exclusion Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Reversed-Phase HPLC -- Glycoprotein Analysis -- Protein Sequencing -- Solid-Phase Peptide Synthesis -- Protein Engineering -- Monoclonal Antibodies -- Phage-Display Libraries -- Enzyme- Linked Immunosorbent Assay (ELISA) -- Epitope Mapping -- lmmunocytochemistry -- Flow Cytometry -- Mass Spectrometry -- The Technique of In Situ Hybridization.
520 _aIn the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully.The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or described non-text content (images, graphs etc). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at accessibilitysupport@springernature.com if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aRapley, Ralph.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWalker, John M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896035010
776 0 8 _iPrinted edition:
_z9781489943545
776 0 8 _iPrinted edition:
_z9781489943552
830 0 _aSpringer Protocols Handbooks,
_x1949-2456
856 4 0 _uhttps://doi.org/10.1007/978-1-59259-642-3
912 _aZDB-2-PRO
999 _c8564
_d8564