000 03716nam a22004575i 4500
001 978-1-59259-180-0
003 DE-He213
005 20260610083722.0
007 cr nn 008mamaa
008 100301s2002 xxu| s |||| 0|eng d
020 _a9781592591800
_9978-1-59259-180-0
024 7 _a10.1385/1592591809
_2doi
050 4 _aRS1-441
072 7 _aMQP
_2bicssc
072 7 _aMED072000
_2bisacsh
072 7 _aMQP
_2thema
082 0 4 _a615.1
_223
245 1 0 _aHigh Throughput Screening
_h[electronic resource] :
_bMethods and Protocols /
_cedited by William P. Janzen.
250 _a1st ed. 2002.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2002.
300 _aX, 265 p. 31 illus., 5 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v190
505 0 _aDesign and Implementation of High Throughput Screening Assays -- Configuring Radioligand Receptor Binding Assays for HTS Using Scintillation Proximity Assay Technology -- Homogeneous Techniques for Monitoring Receptor–Ligand Interactions -- Comparison of SPA, FRET, and FP for Kinase Assays -- Electrochemiluminescence: A Technology Evaluation and Assay Reformatting of the Stat6/P578 Protein–Peptide Interaction -- Cellular Assays in HTS -- Compound Library Management -- Compound Library Management: An Overview of an Automated System -- Natural Products or Not? How to Screen for Natural Products in the Emerging HTS Paradigm -- to Screening Automation -- Unit Automation in High Throughput Screening -- Fully Automated Screening Systems -- Management and Maintenance of High Throughput Screening Systems.
520 _aSince its first use in antibiotic research in the 1940s, High Throughput Screening (HTS) has evolved into a major enabling tool and methodology in the discovery of pharmaceutical, chemical, and agricultural compounds. In High Throughput Screening, leading scientists and researchers expert in molecular discovery explain the diverse technologies and key techniques used in HTS and demonstrate how they can be applied generically. Writing to create precisely the introductory guidebook they wished had been available when they started in HTS, these seasoned authors illuminate the HTS process with richly detailed tutorials on the biological techniques involved, the management of compound libraries, and the automation and engineering approaches needed. Extensive discussions provide readers with all those key elements of pharmacology, molecular biology, enzymology, and biochemistry that will ensure the identification of suitable targets and screens, and detail the technology necessary to mine millions of data points for meaningful knowledge. By presenting accounts not only of the basic principles underlying HTS technology, but also real-use cases, High Throughput Screening constitutes a highly practical introduction to HTS, even as it also provides sufficient detail to work more productively in today's lead generation laboratories.
650 0 _aPharmacy.
650 1 4 _aPharmacy.
700 1 _aJanzen, William P.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896038899
776 0 8 _iPrinted edition:
_z9781489938671
776 0 8 _iPrinted edition:
_z9781489938688
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v190
856 4 0 _uhttps://doi.org/10.1385/1592591809
912 _aZDB-2-PRO
999 _c8540
_d8540