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008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592598700
_9978-1-59259-870-0
024 7 _a10.1385/1592598706
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aMedical BioMethods Handbook
_h[electronic resource] /
_cedited by John M. Walker, Ralph Rapley.
250 _a1st ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aXVI, 644 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Protocols Handbooks,
_x1949-2456
505 0 _aBasic Techniques in Molecular Biology -- Probe Design, Production, and Applications -- Restriction Enzymes -- Southern Blotting as a Diagnostic Method -- Western Blotting as a Diagnostic Method -- Principles and Medical Applications of the Polymerase Chain Reaction -- Single-Strand Conformation Polymorphism (SSCP) Analysis -- Denaturing Gradient Gel Electrophoresis (DGGE) -- Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing -- to Capillary Electrophoresis of DNA -- Mapping Techniques -- Dideoxyfingerprinting for Mutation Detection -- Conformation-Sensitive Gel Electrophoresis -- Amplification Refractory Mutation System and Molecular Diagnostics -- Ligase Chain Reaction -- Chemical Cleavage of Mismatch -- The Protein Truncation Test -- Linkage, Allele Sharing, and Association -- Single-Nucleotide Polymorphisms -- cDNA Microarrays -- Nucleic Acid Sequence-Based Amplification -- Oligonucleotide Ligation Assay -- Quantitative TaqMan Real-Time PCR -- Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine -- Quantitative PCR -- Liquid Chromatography-Mass Spectrometry of Nucleic Acids -- Comparative Genomic Hybridization in Clinical and Medical Research -- Bioinformatic Tools for Gene and Protein Sequence Analysis -- In SituHybridization -- Enzyme-Linked Immunosorbent Assay -- Protein Therapeutics -- Karyotyping -- Microsatellite Analysis -- Analysis of Chromosomal Translocations -- Differential Display -- Techniques for Gene Expression Profiling -- Capillary Electrophoresis in Clinical Analysis -- Flow Cytometry in the Biomedical Arena -- Immunocytochemistry -- Ribotyping in Clinical Microbiology -- Diagnostic Applications of Protein Microarrays -- The Human Genome Project -- Prenatal Diagnosis of Inborn Errors of Metabolism -- Gene Therapy.
520 _aPowerful new techniques in molecular biology have revolutionized our ability to investigate and define cellular processes at the molecular level for medical purposes. In Medical Biomethods Handbook, John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are Southern and Western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy. State-of-the-art and highly practical, Medical Biomethods Handbook offers novice and experienced researchers an in-depth understanding of the theory, practice, and application of the key molecular biology techniques used in biomedical research and diagnostic investigation today. .
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aWalker, John M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRapley, Ralph.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588292889
776 0 8 _iPrinted edition:
_z9781588293343
830 0 _aSpringer Protocols Handbooks,
_x1949-2456
856 4 0 _uhttps://doi.org/10.1385/1592598706
912 _aZDB-2-PRO
999 _c8534
_d8534