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020 _a9781493936649
_9978-1-4939-3664-9
024 7 _a10.1007/978-1-4939-3664-9
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aGap Junction Protocols
_h[electronic resource] /
_cedited by Mathieu Vinken, Scott R. Johnstone.
250 _a1st ed. 2016.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2016.
300 _aXIII, 235 p. 45 illus., 34 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1437
505 0 _aAnalysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction -- DNA Methylation Analysis of Human Tissue-Specific Connexin Genes -- Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis -- Immunohisto- and Cytochemistry Analysis of Connexins -- Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells -- Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor -- Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells -- Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis -- Scrape Loading/Dye Transfer Assay -- Microinjection Technique for Assessment of Gap Junction Function -- Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling -- Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro -- Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging -- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells -- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels -- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.
520 _aPresenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aVinken, Mathieu.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aJohnstone, Scott R.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493936625
776 0 8 _iPrinted edition:
_z9781493936632
776 0 8 _iPrinted edition:
_z9781493981106
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1437
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-3664-9
912 _aZDB-2-PRO
999 _c8531
_d8531