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020 _a9781627033114
_9978-1-62703-311-4
024 7 _a10.1007/978-1-62703-311-4
_2doi
050 4 _aRM300-666
072 7 _aMMG
_2bicssc
072 7 _aMED071000
_2bisacsh
072 7 _aMKG
_2thema
082 0 4 _a615
_223
245 1 0 _aTarget Identification and Validation in Drug Discovery
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Jurgen Moll, Riccardo Colombo.
250 _a1st ed. 2013.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2013.
300 _aXV, 371 p. 62 illus., 19 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029
505 0 _aThe Path to Oncology Drug Target Validation – an Industry Perspective -- Identification of Aptamers as Specific Binders and Modulators of Cell-Surface Receptor Activity -- The Design and Structure-functional Properties  of DNA-based Immunomodulatory Sequences -- siRNA Design Principles and Off-target Effects -- Western Blot Evaluation of siRNA Delivery by pH Responsive Peptides -- High Throughput RNAi Screening for the Identification of Novel Targets -- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development -- CellProfiler and KNIME: Open Source Tools for High Content Screening -- PARP Inhibition as a Prototype for Synthetic Lethal Screens -- Structure-Based Target Druggability Assessment -- Validating Pharmacological Disruption of Protein-protein Interactions by Acceptor Photobleaching FRET Imaging -- Systematic Analysis of Complex Signal Transduction Pathways using Protein Fragment Complementation Assays -- Reverse-Phase Protein Microarrays and Their Utility in Drug Development -- A Cell Culture System that Mimics Chronic Lymphocytic Leukemia Cells Microenvironment for Drug Screening and Characterization -- 2-Dimensional vs. 3-Dimensional in vitro Tumor Migration and Invasion Assays -- Tumor Spheroid-based Migration Assays for Evaluation of Therapeutic Agents -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs).-Genetically Engineered Animal Models for in vivo Target Identification and Validation in Oncology -- Target Validation in Mice by Constitutive andConditional RNAi -- In vivo Target Validation by Inducible RNAi in Human Xenograft Mouse Models -- Brightfield In Situ Hybridization Methods to Discover Gene Amplifications and Rearrangements in Clinical Samples -- Combined microRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers.
520 _aThe major reason for the elevated costs of drug development in the pharmaceutical industry is the high attrition rate. In Target Identification and Validation in Drug Discovery: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to identify and validate new target. These include methods and approaches covering biochemical, cell based, in vivo models and translational methods. Chapters also include selected case reports that demonstrate the integration of these technologies to real life experiences and to demonstrate the multiple use of more than one technology to increase knowledge on a specific target. These Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.   Thorough and intuitive, Target Identification and Validation in Drug Discovery: Methods and Protocols contains a comprehensive list of essential methods and clear protocols to follow.
650 0 _aPharmacology.
650 0 _aMedicinal chemistry.
650 1 4 _aPharmacology.
650 2 4 _aMedicinal Chemistry.
700 1 _aMoll, Jurgen.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aColombo, Riccardo.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627033107
776 0 8 _iPrinted edition:
_z9781627033121
776 0 8 _iPrinted edition:
_z9781493962785
830 0 _aMethods in Molecular Biology,
_x1940-6029
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-311-4
912 _aZDB-2-PRO
999 _c8530
_d8530