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020 _a9781603271752
_9978-1-60327-175-2
024 7 _a10.1007/978-1-60327-175-2
_2doi
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_2bicssc
072 7 _aSCI017000
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082 0 4 _a571.6
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245 1 0 _aInositol Phosphates and Lipids
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Christopher J. Barker.
250 _a1st ed. 2010.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2010.
300 _aX, 230 p. 54 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v645
505 0 _aThe Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells -- HPLC Separation of Inositol Polyphosphates -- Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells -- A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate -- Synthesis of InsP 7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1) -- Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP 7) -- Synthesis and Nonradioactive Micro-analysis of Diphosphoinositol Phosphates by HPLC with Postcolumn Complexometry -- Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio -- Isolation of Inositol 1,4,5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference -- Phosphoinositide-Specific Phospholipase C ?1 Signal Transduction in the Nucleus -- Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides -- Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC -- Phosphoinositide Analysis by Liquid Chromatography–Mass Spectrometry -- Imaging Phosphoinositide Dynamics in Living Cells.
520 _aInositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aBarker, Christopher J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781603271745
776 0 8 _iPrinted edition:
_z9781607616795
776 0 8 _iPrinted edition:
_z9781617796890
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v645
856 4 0 _uhttps://doi.org/10.1007/978-1-60327-175-2
912 _aZDB-2-PRO
999 _c8527
_d8527