000 03981nam a22004455i 4500
001 978-1-59259-873-1
003 DE-He213
005 20260610083722.0
007 cr nn 008mamaa
008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592598731
_9978-1-59259-873-1
024 7 _a10.1385/1592598730
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aImmunochemical Protocols
_h[electronic resource] /
_cedited by Robert Burns.
250 _a3rd ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aX, 318 p. 22 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v295
505 0 _aImmunization Strategies for Antibody Production -- Synthetic Peptides as Antigens for Antibody Production -- Production of Polyclonal Antibodies in Rabbits -- Making Hybridomas -- Growing Hybridomas -- Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments -- Purification of Antibodies and Preparation of Antibody Fragments -- Conjugation of Antibodies to Alkaline Phosphatase -- Conjugation of Antibodies to Horseradish Peroxidase -- Conjugation of Antibodies to Fluorescein or Rhodamine -- Conjugation of Antibodies to Biotin -- Preparation of Colloidal Gold Probes -- Immunogold Probes in Light Microscopy -- Immunoelectron Microscope Techniques in Plant Virus Diagnosis -- Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay -- Competitive Enzyme-Linked Immunosorbent Assay -- Immunoblotting Techniques -- Epitope Mapping -- Generation and Testing of Immunoaffinity Columns -- Immunocapture-Polymerase Chain Reaction -- Magnetic Cell Sorting -- Cell Enrichment and Immunochemical Staining.
520 _aThis new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the major immunochemical techniques currently used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. The authors provide full details of methods for antigen selection and preparation, antibody production, and reagent manufacture. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Immunochemical Protocols, Third Edition, takes both novice and experienced researchers from project initiation to final test format with a broad array of protocols suited to immediate use in the laboratory, as well as methods that can be successfully applied to entirely new systems and processes.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aBurns, Robert.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588292742
776 0 8 _iPrinted edition:
_z9781617374661
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v295
856 4 0 _uhttps://doi.org/10.1385/1592598730
912 _aZDB-2-PRO
999 _c8524
_d8524