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001 978-1-61779-046-1
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020 _a9781617790461
_9978-1-61779-046-1
024 7 _a10.1007/978-1-61779-046-1
_2doi
050 4 _aQD431-431.7
072 7 _aPSBC
_2bicssc
072 7 _aSCI049000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572.6
_223
245 1 0 _aArgonaute Proteins
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Tom C. Hobman, Thomas F. Duchaine.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aXII, 344 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v725
505 0 _aPurification of Native Argonaute Complexes from the Fission Yeast Schizosaccharomyces pombe -- Chromatin Immuno-Precipitation in Fission Yeast -- Biochemical Analyses of Endogenous Argonaute Complexes Immunopurified with Anti-Argonaute Monoclonal Antibodies -- Mapping of Ago2-GW182 Functional Interactions -- Continuous Density Gradients to Study Argonaute and GW182 Complexes Associated with the Endocytic Pathway -- In vitro RISC Cleavage Assay -- Native Gel Analysis for RISC Assembly -- Purification and Assembly of Human Argonaute, Dicer, and TRBP Complexes -- Detection of Human Dicer and Argonaute 2 Catalytic Activity -- Imaging the Cellular Dynamics of Drosophila Argonaute Proteins -- Live Cell Imaging of Argonaute Proteins in Mammalian Cells -- Reporter-Based Assays for Analyzing RNA Interference in Mammalian Cells -- Artificial Tethering of Argonaute Proteins for Studying their Role in Translational Repression of Target mRNAs -- An Efficient System For Let-7 MicroRNA- And GW182 Protein-Mediated Deadenylation In vitro -- Cell-Free microRNA-Mediated Translation Repression in C. elegans -- Argonaute Pull-Down and RISC Analysis Using 2'-O-Methylated Oligonucleotides Affinity Matrices -- Cloning Argonaute-Associated Small RNAs from C. elegans -- Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs -- Generation of an Inducible Mouse ES Cell Line Deficient for Argonaute Proteins -- Whole Cell Proteome Regulation by microRNAs Captured in a Pulsed SILAC Mass Spectrometry Approach.
520 _aWith the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner.  Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels.  In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.
650 0 _aProteins .
650 0 _aBiotechnology.
650 1 4 _aProtein Biochemistry.
650 2 4 _aChemical Bioengineering.
700 1 _aHobman, Tom C.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aDuchaine, Thomas F.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781617790454
776 0 8 _iPrinted edition:
_z9781617790478
776 0 8 _iPrinted edition:
_z9781493958276
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v725
856 4 0 _uhttps://doi.org/10.1007/978-1-61779-046-1
912 _aZDB-2-PRO
999 _c8519
_d8519