000 03653nam a22004455i 4500
001 978-1-59259-955-4
003 DE-He213
005 20260610083722.0
007 cr nn 008mamaa
008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592599554
_9978-1-59259-955-4
024 7 _a10.1385/1592599559
_2doi
050 4 _aQD71-142
072 7 _aPNF
_2bicssc
072 7 _aSCI013010
_2bisacsh
072 7 _aPNF
_2thema
082 0 4 _a543
_223
245 1 0 _aNatural Products Isolation
_h[electronic resource] /
_cedited by Satya D. Sarker.
250 _a2nd ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aXII, 516 p. 133 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Biotechnology,
_x1940-607X ;
_v20
505 0 _aNatural Product Isolation -- Initial and Bulk Extraction -- Supercritical Fluid Extraction -- An Introduction to Planar Chromatography -- Isolation of Natural Products by Low-Pressure Column Chromatography -- Isolation by Ion-Exchange Methods -- Separation by High-Speed Countercurrent Chromatography -- Isolation by Preparative High-Performance Liquid Chromatography -- Hyphenated Techniques -- Purification by Solvent Extraction Using Partition Coefficient -- Crystallization in Final Stages of Purification -- Dereplication and Partial Identification of Compounds -- Extraction of Plant Secondary Metabolites -- Isolation of Marine Natural Products -- Isolation of Microbial Natural Products -- Purification of Water-Soluble Natural Products -- Scale-Up of Natural Product Isolation -- Follow-Up of Natural Product Isolation.
520 _aBuilding on the high reputation of the first edition of Natural Products Isolation, the authors have in this second edition not only significantly updated all the techniques to reflect recent advances in the field, but also added three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. These readily reproducible methods make it possible for even the novice to extract, prepare, and isolate natural products from given source materials. The book includes not only powerful cutting-edge techniques, but also simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Natural Products Isolation, Second Edition, offers the aspiring natural products researcher all the information needed to attain mastery of essential methods and the more experienced investigator a useful reference guide to all the available techniques.
650 0 _aAnalytical chemistry.
650 1 4 _aAnalytical Chemistry.
700 1 _aSarker, Satya D.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588294470
776 0 8 _iPrinted edition:
_z9781617375750
830 0 _aMethods in Biotechnology,
_x1940-607X ;
_v20
856 4 0 _uhttps://doi.org/10.1385/1592599559
912 _aZDB-2-PRO
999 _c8516
_d8516