000 04665nam a22004575i 4500
001 978-1-59259-204-3
003 DE-He213
005 20260610083722.0
007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592592043
_9978-1-59259-204-3
024 7 _a10.1385/0896034437
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aForensic DNA Profiling Protocols
_h[electronic resource] /
_cedited by Patrick J. Lincoln.
250 _a1st ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aX, 309 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v98
505 0 _aRecovery of High-Molecular-Weight DNA from Blood and Forensic Specimens -- Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating Resin -- PCR Analysis of DNA from Fresh and Decomposed Bodies and Skeletal Remains in Medicolegal Death Investigations -- PCR Analysis from Cigaret Butts, Postage Stamps, Envelope Sealing Flaps, and Other Saliva-Stained Material -- Quantification of DNA By Slot-Blot Analysis -- Rapid Assessment of PCR Product Quality and Quantity by Capillary Electrophoresis -- Southern Blotting of Genomic DNA for DNA Profiling -- Preparation and Use of Alkaline-Phosphatase-Conjugated Oligonucleotide Probes for Single-Locus and Multilocus VNTR Analysis -- Preparation and Use of 32P-Labeled Single-Locus VNTR Probes in Identity Testing -- Statistical Methods Employed in Evaluation of Single-Locus Probe Results in Criminal Identity Cases -- Interpretation and Statistical Evaluation of Multilocus DNA Fingerprints in Paternity and Relationship Testing -- Overview of PCR-Based Systems in Identity Testing -- An Introduction to PCR Primer Design and Optimization of Amplification Reactions -- Analysis of Amplified Fragment-Length Polymorphisms (VNTR/STR Loci) for Human Identity Testing -- Amplification of Short Tandem Repeat Loci Using PCR -- Manual Electrophoretic Methods for Genotyping Amplified STR Loci -- 17 Genotyping STR Loci Using an Automated DNA Sequencer -- Separation of PCR Fragments by Means of Direct Blotting Electrophoresis -- 19 Amplification and Sequencing of Mitochondrial DNA in Forensic Casework -- Minisatellite Variant Repeat Unit Mapping Using PCR (MVR-PCR) -- Sex Determination by PCR Analysis of the X-Y Amelogenin Gene -- Species Determination by Analysis of the Cytochrome b Gene -- Use of the AmpliType PM + HLA DQA1 PCR Amplification and Typing Kits for Identity Testing -- The Use of Capillary Electrophoresis in Genotyping STR Loci -- Solid-Phase Minisequencing as a Tool to Detect DNA Polymorphism.
520 _aIn Forensic DNA Profiling Protocols, leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR-based test systems, the now widely used STR typing systems (complete with details of both manual and automated detection systems), repeat unit mapping (MVR), direct-phase minisequencing, capillary electrophoresis, and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood, semen, skeletal remains, and saliva), species testing, sex determination, and mitochondrial DNA testing. Forensic DNA Profiling Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science. The readily reproducible methods elucidated here represent the state-of-the-art for those scientists who want to introduce, establish, and perfect DNA identification techniques in their laboratories.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aLincoln, Patrick J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896034433
776 0 8 _iPrinted edition:
_z9781617370380
776 0 8 _iPrinted edition:
_z9781489942562
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v98
856 4 0 _uhttps://doi.org/10.1385/0896034437
912 _aZDB-2-PRO
999 _c8502
_d8502