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020 _a9781597455268
_9978-1-59745-526-8
024 7 _a10.1007/978-1-59745-526-8
_2doi
050 4 _aRM300-666
072 7 _aMMG
_2bicssc
072 7 _aMED071000
_2bisacsh
072 7 _aMKG
_2thema
082 0 4 _a615
_223
245 1 0 _aPotassium Channels
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Jonathan D. Lippiat.
250 _a1st ed. 2009.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2009.
300 _aX, 302 p. 59 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v491
505 0 _aMethods For Studying Potassium Channel Transcription And Mrna Splicing -- Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA—Protein Interactions by Chromatin Immunoprecipitation -- Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries -- Cloning of Potassium Channel Splice Variants from Tissues and Cells -- Methods for Studying the Cell Biology of Potassium Channel Proteins -- Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KATP Channels -- Investigation of KATP Channel Endocytosis by Immunofluorescence -- Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting -- Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts -- Determination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein—Lipid Overlay Assay -- Protein Complex Analysis of Native Brain Potassium Channels by Proteomics -- Electrophysiological Techniques For The Study Of Potassium Channel Function -- Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique -- Whole-Cell Recording Using the Perforated Patch Clamp Technique -- Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration -- Planar Patch Clamp: Advances in Electrophysiology -- Analysing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers -- Optical Techniques For The Study Of Potassium Channel Function And Interactions -- Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly -- The Use of FRET Microscopy to ElucidateSteady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels -- The Voltage-Clamp Fluorometry Technique -- Methods For Studying Potassium Channelopathies And Pharmacological Modulators -- Identification of Mutations in the Kir6.2 Subunit of the KATP Channel -- Modulation of Potassium Ion Channel Proteins Utilising Antibodies -- Fluorescence-Based Tl+?Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+?Activated K+ Channel Modulators -- Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties -- Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-Clamp Technique.
520 _aDue to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols, experts in the field present a range of experimental approaches that have been developed to investigate potassium channel structure, function, pharmacology, cell biology, gene expression, and their role in disease. Many of these techniques study potassium channels as cellular proteins as well as the resultant membrane biophysics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section, which highlights tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Potassium Channels: Methods and Protocols provides a wide range of approaches in order to aid current research projects and inspire new investigations.
650 0 _aPharmacology.
650 0 _aCytology.
650 0 _aBiological transport.
650 0 _aCell membranes.
650 1 4 _aPharmacology.
650 2 4 _aCell Biology.
650 2 4 _aMembrane Trafficking.
700 1 _aLippiat, Jonathan D.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781934115657
776 0 8 _iPrinted edition:
_z9781603279833
776 0 8 _iPrinted edition:
_z9781617379406
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v491
856 4 0 _uhttps://doi.org/10.1007/978-1-59745-526-8
912 _aZDB-2-PRO
999 _c8474
_d8474