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020 _a9781071647066
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024 7 _a10.1007/978-1-0716-4706-6
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082 0 4 _a573.1
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245 1 0 _aMethods in Cardiovascular and Lymphatic Biology
_h[electronic resource] /
_cedited by Evangelia Papadimitriou, Constantinos M. Mikelis.
250 _a1st ed. 2025.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2025.
300 _aXIII, 191 p. 54 illus., 48 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bPDF/UA-1
_2onix
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
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347 _atext file
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490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2956
505 0 _aIsolation and purification of murine lung endothelial cells -- Two-step Positive Selection Method for the Magnetic Isolation of Lymphatic Endothelial Cells -- Isolation and Enumeration of Circulating Endothelial Cells with Antibody Labeled Staggered Herringbone Microfluidic System -- A Guided Approach to Establish a Functional Humanized Brain-on-Chip Microfluidic Model of the Neurovascular System -- Human iPSC-derived astrocyte-endothelial cell and neuron-endothelial cell co-culture models of the blood-brain barrier to study the impact of ischemic stroke in vitro -- Isolation of rodent aortic smooth muscle cells by enzymatic digestions -- Studying the vascular tone of mouse aortic rings in a standard wire myograph -- Unveiling Central Nervous System Pericytes: Immunofluorescence Protocol and Confocal Microscopy -- Serum-free Mouse Aortic Ring Assay of Angiogenesis -- A New Method to Visualize Retinal Deep Vascular Layers Using Wholemount Tissue Preparations -- Live calcium imaging on mouse primary brain endothelial cells -- Studying nitric oxide in endothelial cells -- Identifying protein-protein interaction or protein phosphorylation by Proximity Ligation Assay (PLA) in endothelial cells -- Label-free imaging with automated tracking and cell-by-cell analysis of migration, morphology, and differentiation of endothelial cells -- Assessment of angiogenic properties of malignant pleural effusion (MPE) fluid -- Ultrasound Contrast Agents in Mice: Bridging the Gap from Preclinical Research to Clinical Translation.
520 _aThis volume provides an overview of advanced protocols for vascular biology research, comprising cardiovascular and lymphatic biology. Chapters detail techniques for isolating and characterizing endothelial and mural cell populations, disseminating signaling pathways and protein-protein interactions, constructing organ-on-chip systems, and using animal models and techniques with bench-to-bedside potential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Methods in Cardiovascular and Lymphatic Biology aims to provide protocols and applications that can be used by both new investigators and experienced professionals to advance cardiovascular and lymphatic research.
532 8 _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCardiovascular system.
650 0 _aPhysiology.
650 0 _aLymphatics.
650 0 _aBiological models.
650 1 4 _aCardiovascular Physiology.
650 2 4 _aLymphatic System.
650 2 4 _aBiological Models.
700 1 _aPapadimitriou, Evangelia.
_eeditor.
_0(orcid)0000-0001-6429-4325
_1https://orcid.org/0000-0001-6429-4325
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_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMikelis, Constantinos M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071647059
776 0 8 _iPrinted edition:
_z9781071647073
776 0 8 _iPrinted edition:
_z9781071647080
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2956
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-4706-6
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