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020 _a9781071645918
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024 7 _a10.1007/978-1-0716-4591-8
_2doi
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082 0 4 _a571.96028
_223
245 1 0 _aEpitope Mapping Protocols
_h[electronic resource] /
_cedited by Johan Rockberg, Johan Nilvebrant, Magdalena Malm, Niklas Berndt Thalén.
250 _a4th ed. 2025.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2025.
300 _aXI, 394 p. 84 illus., 78 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bPDF/UA-1
_2onix
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2937
505 0 _aAn Overview of Epitopes and Methods for Antibody Epitope Mapping -- Antibody-Antigen Epitope Mapping by X-Ray Crystallography -- Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations -- Cryo-Electron Microscopy-Based Epitope Mapping -- Epitope Mapping Using Yeast Display and Next Generation Sequencing -- Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing -- High-Density Antigen Microarrays for the Assessment of Antibody Selectivity -- Mammalian Cell Display with Automated Oligo Design and Library Assembly Allows for Rapid Residue Level Conformational Epitope Mapping -- Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries -- Mapping of Antibody Structural Epitopes Using Bacterial Cell Surface Display of Folded Domains -- Highly Multiplexed Immunoassays for the Validation of Anti-GPCR Antibodies -- Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-Linkers -- Genetic Code Expansion to Enable Site-Specific Post-Translational Peptide Epitope Tagging of G Protein-Coupled Receptors in Live Cells -- Multiplexed Epitope Mapping of HIV-1 Neutralizing Antibodies by Coupling Chemical Labeling of Cysteines to Deep Sequencing -- Predicting Epitopes of Synthetic Binding Proteins Using Protein Structure Prediction and Protein-Protein Docking -- Structure-Based Epitope Profiling with the Structural Profiling of Antibodies to Cluster by Epitope 2 (SPACE2) Algorithm -- Binding Kinetics and Epitope Binning Using Surface Plasmon Resonance -- High-Throughput Epitope Binning Analysis of Human Monoclonal Antibodies to Assess Epitope Diversity Using the Octet Bio-Layer Interferometry Platform -- Competitive Epitope Binning Using HT-SPR -- Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors -- B Cell Epitope Mapping by High Density Competition ELISAs.
520 _aThis detailed new edition explores methodologies for the characterization of antibody-antigen interactions tailored to different applications and requirements. Beginning with an overview of key concepts and definitions related to antibody epitopes, the book continues with sections on structural biology methods, display technologies to analyze libraries of proteins or protein arrays, techniques based on protein labeling, as well as computational approaches and binding assays that rely on binding interactions or competition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Epitope Mapping Protocols, Fourth Edition serves as an ideal resource for scientists from a wide range of fields involved in critical antibody research. Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
532 8 _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiology
_xTechnique.
650 0 _aExperimental immunology.
650 0 _aPharmacology.
650 1 4 _aImmunological Techniques.
650 2 4 _aPharmacology.
700 1 _aRockberg, Johan.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aNilvebrant, Johan.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMalm, Magdalena.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aThalén, Niklas Berndt.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071645901
776 0 8 _iPrinted edition:
_z9781071645925
776 0 8 _iPrinted edition:
_z9781071645932
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2937
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-4591-8
912 _aZDB-2-PRO
999 _c8431
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