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| 001 | 978-1-0716-4574-1 | ||
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| 005 | 20260610083720.0 | ||
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| 008 | 250526s2025 xxu| s |||| 0|eng d | ||
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_a9781071645741 _9978-1-0716-4574-1 |
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_a10.1007/978-1-0716-4574-1 _2doi |
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_aPSAK1 _2bicssc |
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_aDNA Damage Detection _h[electronic resource] : _bMethods and Protocols / _cedited by Takamitsu A. Kato. |
| 250 | _a1st ed. 2025. | ||
| 264 | 1 |
_aNew York, NY : _bSpringer US : _bImprint: Humana, _c2025. |
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_aX, 150 p. 31 illus., 21 illus. in color. _bonline resource. |
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_atext _btxt _2rdacontent |
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_acomputer _bc _2rdamedia |
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_aonline resource _bcr _2rdacarrier |
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_bTable of contents navigation _2onix |
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_bSingle logical reading order _2onix |
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_bShort alternative textual descriptions _2onix |
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_bUse of color is not sole means of conveying information _2onix |
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_bUse of high contrast between text and background color _2onix |
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_bNext / Previous structural navigation _2onix |
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_bAll non-decorative content supports reading without sight _2onix |
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_atext file _bPDF _2rda |
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_aMethods in Molecular Biology, _x1940-6029 ; _v2933 |
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| 505 | 0 | _aModified Alkaline Comet Assay to Detect Oxidative DNA Damage -- Radiation-Induced DNA Strand Break Detection by Gel Electrophoresis -- Quantitative Measurement of 8-Hydroxy-2′-Deoxyguanosine in Serum/Plasma and Urine Using an Enzyme-Linked Immunosorbent Assay -- Radiation-Induced Gamma-H2AX Foci Staining and Analysis -- Non-Radiolabeled Radioresistant DNA Synthesis and S-Phase Checkpoint Analysis -- Constant Field Gel Electrophoresis Analysis for DNA Double-Strand Break and Its Repair -- Detection of Chromatid Break and Micronucleus Formation Induced by Low- and High-LET Irradiation -- Measurement of DNA Polymerase and Microhomology-Mediated End-Joining Activities by Gel Electrophoresis and smFRET Techniques -- UV-Induced DNA Damage Detection by ELISA Analysis -- G2 Premature Chromosome Condensation (G2-PCC) by Calyculin A to Analyze DNA Double-Strand Breaks and Their Repair -- Mitotic Shake-Off and Cell Cycle Synchronization -- DCFDA Assay for Oxidative Stress Measurement in Fluorometer -- HPRT Mutation Assay for Chinese Hamster Ovary Cells -- Detection of De Novo Mutations by Sequencing Reduced Representation Libraries -- Chemical-Induced DNA Damage Identification by High-Throughput Detection of DNA Double-Strand Break -- Assessing DNA Damage Through the Cell Cycle Using Flow Cytometry -- A Mass Spectrometry-Based Proteomics Workflow for Concurrent Profiling of Protein Thiol Oxidation and Phosphorylation -- Analyzing Homologous Recombination Using Antibiotic Marker Substrates in Mammalian Cells. | |
| 520 | _aThis volume explores techniques in DNA damage analysis, important not only for understanding cellular health after exposure to genotoxic agents but also for assessing cellular ability of genome maintenance. The book explores traditional DNA damage measurement methods, as well as modern fluorescence-based analysis and high throughput automatic analysis methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and timely, DNA Damage Detection: Methods and Protocols serves as an ideal guide for readers who are unfamiliar with DNA damage as well as experts seeking protocols to adapt to their own research. | ||
| 532 | 8 | _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. | |
| 532 | 8 | _aNo reading system accessibility options actively disabled | |
| 532 | 8 | _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com | |
| 650 | 0 | _aDNA damage. | |
| 650 | 0 | _aDNA repair. | |
| 650 | 0 | _aGenetics. | |
| 650 | 1 | 4 | _aDNA Damage and Repair. |
| 650 | 2 | 4 | _aGenetics and Genomics. |
| 700 | 1 |
_aKato, Takamitsu A. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071645734 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071645758 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071645765 |
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_aMethods in Molecular Biology, _x1940-6029 ; _v2933 |
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| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-1-0716-4574-1 |
| 912 | _aZDB-2-PRO | ||
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_c8420 _d8420 |
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