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020 _a9781071644188
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024 7 _a10.1007/978-1-0716-4418-8
_2doi
050 4 _aRM300-666
072 7 _aMMG
_2bicssc
072 7 _aMED071000
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072 7 _aMKG
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082 0 4 _a615
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245 1 0 _aTarget Identification and Validation in Drug Discovery
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Jürgen Moll, Sebastian Carotta.
250 _a3rd ed. 2025.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2025.
300 _aX, 270 p. 71 illus., 63 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2905
505 0 _aA Systemic View of Target Identification: Modeling the Warburg Effect -- Structural Biology for Target Identification and Validation -- Design and Preliminary Screen of Antisense Oligonucleotides -- Integration of RNAi and Small Molecule Library Screens to Identify Targets for Drug Discovery -- A Robust siRNA Screening Approach with Optimized Conditions for Large-Scale Transfection in Multiple Human Cancer Cell Lines -- Functional Lineage Tracing Using CaTCH -- Exploring the Perturbation of Biological Processes Caused by Gene Upregulation Using Knock-In Transfection, Transcriptomics, and Bioinformatics Analysis -- A Reverse Engineering Approach to Optimize Chemical Synergy Between Target and Phenotype: Bridging the Cancer and Malaria Indications -- High-Content Imaging-Based Screening for Anti-Cancer Compounds in Zebrafish Xenografts -- Identification of Protein Kinase Drug Targets Using Activity Estimation in Clinical Phosphoproteomics -- Click Chemistry for Target Engagement Studies -- The Power of Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS) in Structural Biology and Protein-Ligand Interactions -- Advancing Target Validation and Ligandability Assessment: Fragment Screening via 19F NMR Spectroscopy -- In Vivo Micro-CT Imaging for Quantitative Longitudinal Assessment of Pulmonary Diseases in Small Animals -- The NeuroSphere Assay Applied to Neural Stem Cells and Cancer Stem Cells -- High Efficiency Lentiviral Transduction of Colon Organoids Using Reversible 2D/3D Culture Techniques -- 3D Tumor Model to Study Immune Cell Infiltration.
520 _aThis third edition book explores breakthrough techniques in the field of drug target identification and validation. With technologies and methodologies ranging from target identification to the analysis of clinical samples, as well as newer areas like artificial intelligence and machine learning applications, the book will aid decision-makers in drug development by offering a succinct overview of the available technologies for portfolio management. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Target Identification and Validation in Drug Discovery: Methods and Protocols, Third Edition will help a diverse audience of scientists find valuable insights to address their drug development inquiries.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aPharmacology.
650 0 _aMolecular biology.
650 1 4 _aPharmacology.
650 2 4 _aMolecular Biology.
700 1 _aMoll, Jürgen.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aCarotta, Sebastian.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071644171
776 0 8 _iPrinted edition:
_z9781071644195
776 0 8 _iPrinted edition:
_z9781071644201
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2905
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-4418-8
912 _aZDB-2-PRO
999 _c8409
_d8409