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024 7 _a10.1007/978-1-0716-4426-3
_2doi
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245 1 0 _aOncogene-Induced Senescence
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Vassilis G. Gorgoulis, Maria Cavinato, Konstantinos Evangelou.
250 _a1st ed. 2025.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2025.
300 _aXIII, 363 p. 57 illus., 54 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2906
505 0 _aIn Situ and In Silico Methods for Senescence Identification in Human Liver Diseases -- Current Methodologies to Assess Cellular Senescence in Cancer -- Selective Sorting of Senescent Cell Subpopulations Compatible with Downstream Genomics Pipelines -- Detection and Characterization of Senescent Cells with Flow Cytometry -- Detecting Senescence in T Cells by Flow Cytometry Using the SA-β-galactosidase Assay -- The DNA Damage Response as an Auxiliary Indicator of Senescence in Cancer: A User-Friendly Toolkit of Markers and Detection Methods -- Precise Mapping of Physiological DSBs Using In-Suspension Break Labelling In Situ and Sequencing (sBLISS) -- Detecting Telomeric DNA Damage by Immuno-Telo FISH -- Combined In Situ Transcriptomic and Immunofluorescence to Assess the Effects on DNA Damage and Innate Immune Responses Induced by Bacterial Genotoxin in In Vivo Models -- Two-Replication Round Telomere Strand-Specific Chromosome Orientation Fluorescent In Situ Hybridization -- Identification of Oncogene-Induced Senescence-Associated MicroRNAs -- Global Proteomic Profiling to Unravel Lipofuscin’s Protein Repertoire -- Electron Microscopy to Visualize and Quantify Mitochondrial Structure and Organellar Interactions in Cultured Cells during Senescence -- Isolation and Characterization of EVs Containing Mitochondria from OIS Cells -- Isolation and Characterization of Endothelial Colony Forming Cells (ECFC): Studying Endothelial Senescence for Translational Studies and for Personalized Medicine -- In Vitro Modeling of Vascular Senescence -- Studying Cellular Senescence Using the Model-Organism Drosophila melanogaster -- C. elegans as a Screening Platform for Anti-Aging Compounds -- Natural Product-Derived Senotherapeutics: Extraction and Biological Evaluation Techniques.
520 _aThis volume explores a collection of the most recent experimental approaches for the detection, modelling, characterization, and in-depth computational analysis of the biological phenomenon known as Oncogene-Induced Senescence (OIS). The chapters in this book cover topics such as protocols to study OIS in two-dimensional, three-dimensional, and in vivo (animal and human) settings; analyzing OIS using high-throughput bioinformatics; and discriminate OIS from aging. Published in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting to avoid known pitfalls. Comprehensive and authoritative, Oncogene-Induced Senescence: Methods and Protocols is a valuable resource for basic researchers and clinicians in the oncology field, and a great guide for computational scientists.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCancer
_xGenetic aspects.
650 0 _aCytology.
650 0 _aAging.
650 1 4 _aCancer Genetics and Genomics.
650 2 4 _aCellular Senescence.
700 1 _aGorgoulis, Vassilis G.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aCavinato, Maria.
_eeditor.
_0(orcid)0000-0002-4891-9636
_1https://orcid.org/0000-0002-4891-9636
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aEvangelou, Konstantinos.
_eeditor.
_0(orcid)0000-0002-2462-5748
_1https://orcid.org/0000-0002-2462-5748
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071644256
776 0 8 _iPrinted edition:
_z9781071644270
776 0 8 _iPrinted edition:
_z9781071644287
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2906
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-4426-3
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