000 03668nam a22004575i 4500
001 978-1-59259-683-6
003 DE-He213
005 20260610083720.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592596836
_9978-1-59259-683-6
024 7 _a10.1385/1592596835
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aNMDA Receptor Protocols
_h[electronic resource] /
_cedited by Min Li.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _aX, 196 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v128
505 0 _aIsolation of Receptor Clones by Expression Screening in Xenopus Oocytes -- Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors -- Transient Expression of Functional NMDA Receptors in Mammalian Cells -- Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells -- Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells -- Immunocytochemistry of NMDA Receptors -- The Use of Ligand Binding in Assays of NMDA Receptor Function -- Calmodulin Modification of NMDA Receptors -- Detergent Solubilization and Immunoprecipitation of Native NMDA Receptors -- Redox Sensitivity of NMDA Receptors -- In Vitro Selection of Peptides Acting on NMDA Receptors -- NMDA Receptor Pharmacology and Analysis of Patch-Clamp Recordings -- Determination of Membrane Topology of Glutamate Receptors -- Engineering the NMDA Receptor Channel Lining -- High-Throughput Functional Detection of NMDA Receptor Activity.
520 _aIn NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane receptors or signaling receptors. NMDA Receptor Protocols provides today's scientists at all skill levels a diverse collection of highly reproducible molecular techniques for ion channel research and biotherapeutic development. Eminently practical and reproducible, these techniques offer powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aLi, Min.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896037137
776 0 8 _iPrinted edition:
_z9781617371462
776 0 8 _iPrinted edition:
_z9781489943613
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v128
856 4 0 _uhttps://doi.org/10.1385/1592596835
912 _aZDB-2-PRO
999 _c8388
_d8388