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020 _a9781493904105
_9978-1-4939-0410-5
024 7 _a10.1007/978-1-4939-0410-5
_2doi
050 4 _aQR180-189.5
072 7 _aMJCM
_2bicssc
072 7 _aMED044000
_2bisacsh
072 7 _aMJCM
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082 0 4 _a571.96
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082 0 4 _a616.079
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245 1 0 _aDNA Vaccines
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Monica Rinaldi, Daniela Fioretti, Sandra Iurescia.
250 _a3rd ed. 2014.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2014.
300 _aXI, 308 p. 43 illus., 32 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1143
505 0 _aA Blueprint for DNA Vaccines Design -- Enhancement of Plasmid-Mediated Transgene Expression -- Strategies for Improving DNA Vaccines Performance -- Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies -- Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies -- Development of Antibiotic-Free Selection System for Safer DNA Vaccination -- Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin -- DNA Vaccination in Skin Enhanced by Electroporation -- Intradermal Vaccination by DNA Tattooing -- Microneedle Applications for DNA Vaccine Delivery to the Skin -- Multivalent DNA-Based Vectors for DNA Vaccines Delivery -- Superparamagnetic Nanoparticles Delivery of DNA Vaccine -- Plasmid Fermentation Process for DNA Immunization Applications -- Pharmaceutical Grade Large Scale Plasmid DNA Manufacturing Process -- Protective and Therapeutic DNA Vaccination Against Allergic Diseases -- Immunotherapy for Alzheimer’s Disease: DNA- and Protein-Based Epitope Vaccines -- Tetravalent DNA Vaccine Product as a Vaccine Candidate Against Dengue -- DNA Vaccination as a Treatment for Chronic Kidney Disease.
520 _aDNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.  Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.  Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.
650 0 _aImmunology.
650 0 _aBiotechnology.
650 0 _aPharmacology.
650 1 4 _aImmunology.
650 2 4 _aChemical Bioengineering.
650 2 4 _aPharmacology.
700 1 _aRinaldi, Monica.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aFioretti, Daniela.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aIurescia, Sandra.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493904099
776 0 8 _iPrinted edition:
_z9781493904112
776 0 8 _iPrinted edition:
_z9781493946532
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1143
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-0410-5
912 _aZDB-2-PRO
999 _c8373
_d8373