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020 _a9781071650561
_9978-1-0716-5056-1
024 7 _a10.1007/978-1-0716-5056-1
_2doi
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_2bicssc
072 7 _aSCI007000
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072 7 _aPSF
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082 0 4 _a571.96
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245 1 0 _aMacrophage Migration Inhibitory Factor
_h[electronic resource] :
_bMethods and Protocols /
_cedited by James Harris, Timothy W. Muusse.
250 _a2nd ed. 2026.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2026.
300 _aXII, 296 p. 93 illus., 83 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bPDF/UA-1
_2onix
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v3002
505 0 _aExpression and Purification of Recombinant Macrophage Migration Inhibitory Factor (MIF) -- Generation of MIF Peptides via Solid Phase Peptide Synthesis -- Expression and Purification of Recombinant D-Dopachrome Tautomerase (D-DT) -- Structural Determination and Ligand Interaction of Macrophage Inhibitory Factor: A Standardized X-Ray Crystallography Approach -- Generation of High-Quality D-Dopachrome Tautomerase Crystals for Structural Studies -- Fragment Screening of MIF by Surface Plasmon Resonance -- Detection of Noncovalent Protein-Ligand Complexes of D-DT Variants by MALDI-TOF Mass Spectrometry -- Analysis of D-Dopachrome Tautomerase Conformational Flexibility Through Molecular Dynamics Solutions -- MIF NMR Chemical Shift Perturbation Mapping -- Assessing the Tautomerase Activity of MIF Using L-Dopachrome -- Using 4-HPP and PP as Substrates to Assess Purified MIF and D-DT Tautomerase Activity and Inhibition -- Protocols for Assessing the Nuclease Activity of MIF -- Detection of Oxidized Macrophage Migration Inhibitory Factor (oxMIF) in Biological Samples Using Enzyme-Linked Immunosorbent Assay (ELISA) -- Detection of Oxidized Macrophage Migration Inhibitory Factor (oxMIF) in Tissue Samples Using Immunohistochemistry (IHC) -- Exploring the Chemotactic Effects of MIF on Neutrophils and T Cells and Methods for Selective Receptor Targeting -- Polarization of Bone Marrow-Derived Macrophages from Wild-Type and High Human MIF-Expressing CATT7 Polymorphic Mice -- Using Luciferase Reporter Cells to Assess the Effects of MIF Inhibitors on NF-kB Activation -- Monitoring Inflammasome Activity through ASC Speck Formation in THP-1 ASC-GFP Cells -- Small Interfering RNA (siRNA)-Mediated Knockdown of Macrophage Migration Inhibitory Factor Transcript in Adherent and Suspension Cultures of Human Cell Lines -- Stable Transfection of CD74 Gene Variants into A549 Lung Adenocarcinoma Cell Line -- Methods to Determine the Role of MIF in Cilia Biogenesis -- Modelling Fungal and Amoebic Infections: Co-Culture Approaches to Study MIF’s Role in Infection Induced Inflammatory Responses -- Immunofluorescent Quantification of MIF in Larval Zebrafish Tailfin Transfection.
520 _aThis second edition volume expands on the previous edition with new protocols that offer biochemical, biophysical, and biological assays for studying macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT). The chapters in this book cover topics such as generation of MIF peptides via solid phase peptide synthesis; fragment screening of MIF by surface plasmon resonance; MIF NMR chemical shift perturbation mapping; methods to determine the role of MIF in cilia biogenesis; and immunofluorescent quantification of MIF in larval zebrafish tailfin transfection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Macrophage Migration Inhibitory Factor: Methods and Protocols, Second Edition is a valuable resource for any researcher hoping to contribute to the ever expanding world of MIF biology.
532 8 _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCytokines.
650 0 _aBiology
_xTechnique.
650 0 _aExperimental immunology.
650 1 4 _aCytokines and Growth Factors.
650 2 4 _aImmunological Techniques.
700 1 _aHarris, James.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMuusse, Timothy W.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071650554
776 0 8 _iPrinted edition:
_z9781071650578
776 0 8 _iPrinted edition:
_z9781071650585
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v3002
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-5056-1
912 _aZDB-2-PRO
999 _c8363
_d8363