000 06072nam a22006015i 4500
001 978-1-0716-4168-2
003 DE-He213
005 20260610083718.0
007 cr nn 008mamaa
008 241111s2025 xxu| s |||| 0|eng d
020 _a9781071641682
_9978-1-0716-4168-2
024 7 _a10.1007/978-1-0716-4168-2
_2doi
050 4 _aQR
072 7 _aPSG
_2bicssc
072 7 _aSCI045000
_2bisacsh
072 7 _aPSG
_2thema
082 0 4 _a579
_223
245 1 0 _aSchizosaccharomyces pombe
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Ruben C. Petreaca.
250 _a1st ed. 2025.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2025.
300 _aXII, 365 p. 65 illus., 38 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2862
505 0 _aDual Luciferase Reporter Assay in Schizosaccharomyces pombe -- High-Throughput Measurement of Single Fission Yeast Cell Volume Using Fluorescence Exclusion -- Measuring Cell Dimensions in Fission Yeast Using Machine Learning -- Ultrastructure Expansion Microscopy (U-ExM) of the Fission Yeast S. pombe -- Live Imaging of Fission Yeast Single Cell Lineages Using a Microfluidic Device -- Analysis of Cell Wall Mechanics in Fission Yeast -- Pil1 Co-Tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe -- Bi-Molecular Fluorescence Complementation as a Tool to Study Specific Dynamic Interactions Between Proteins in Fission Yeast -- Mapping Active RNA Polymerases in Proliferating and Quiescent Fission Yeast Cells Using Precision Run-On Sequencing -- Fast Fission Yeast Genome Editing by CRISPR/Cas9 Using Gap Repair and Fluoride Selection -- CRISPR–Cas9 Genome Editing in Auxotrophic and Non-Auxotrophic Fission Yeast Strains -- Proliferation Analyses of Conditional Knockdown Strains Using CRISPR Interference in Fission Yeast -- SIMPLE Method for Plasmid Editing -- Investigating Fission Yeast Mutagenesis Using Canavanine Sensitivity Assays -- A Rapidly Inducible DNA Double-Strand Break to Monitor Telomere Formation, DNA Repair, and Checkpoint Activation -- Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome -- Assessing Drug Sensitivity in Fission Yeast Using Half-Maximal Inhibitory Concentration (IC50) Assays -- ChIPmentation for Epigenomic Analysis in Fission Yeast -- Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays -- Measuring Meiotic Recombination Frequency in Schizosaccharomyces pombe Using an Engineered Genetic Interval -- Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe -- Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag -- Identifying Drug Sensitivities in Fission Yeast by Assessing Growth Kinetics -- An “In Schizo” Evaluation System to Screen for Human Kinesin-5 Inhibitors -- Automated Machine Learning Tools to Build Regression Models for Schizosaccharomyces pombe Omics Data.
520 _aThis detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and more. The volume also includes chapters with a focus on computational methods specific to the approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Schizosaccharomyces pombe: Methods and Protocols serves as the ideal guide for researchers in the fission yeast community working with emerging technologies.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMicrobiology.
650 0 _aBiological models.
650 1 4 _aMicrobiology.
650 2 4 _aBiological Models.
700 1 _aPetreaca, Ruben C.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071641675
776 0 8 _iPrinted edition:
_z9781071641699
776 0 8 _iPrinted edition:
_z9781071641705
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2862
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-4168-2
912 _aZDB-2-PRO
999 _c8324
_d8324