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020 _a9781071639344
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024 7 _a10.1007/978-1-0716-3934-4
_2doi
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072 7 _aSCI013010
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082 0 4 _a543.65
_223
245 1 0 _aMass Spectrometry Based Single Cell Proteomics
_h[electronic resource] /
_cedited by Akos Vegvari, Jaakko Teppo, Roman A. Zubarev.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXII, 242 p. 55 illus., 53 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2817
505 0 _aLabel-Free Sample Preparation for Single Cell Proteomics -- Infrared Laser Ablation and Capture of Biological Tissue -- Comprehensive Micro-SPE-Based Bottom-Up Proteomic Workflow for Sensitive Analysis of Limited Samples -- A Sample Preparation Procedure for Isobaric Labelling-Based Single Cell Proteomics -- Nanoliter-Scale Sample Preparation for Single Cell Proteomic Analysis Using Glass-Oil-Air-Droplet Chip -- Fully Integrated Online Strategy for Highly Sensitive Proteome Profiling -- Efficient and Sensitive Sample Preparation, Separations, and Data Acquisition for Label-Free Single-Cell Proteomics -- Robust Surfactant-Assisted One-Pot Sample Preparation for Label-Free Single-Cell and Nanoscale Proteomics -- Spatial Proteomics of Single Hepatocytes with Multiplexed Data-Independent Acquisition (mDIA) -- Profiling Mouse Brain Single-Cell-Type Proteomes via Adeno-Associated Virus-Mediated Proximity Labeling and Mass Spectrometry -- EquiCP: Targeted Single Cell Proteomics by Mass Spectrometry with Isobaric Labeled Multiplexing -- Analyzing Post-Translational Modifications in Single Cells -- Single Cell Proteomics Analysis with Tecan Uno and SCREEN Workflow -- Standardized Workflow for Mass Spectrometry-Based Single-Cell Proteomics Data Processing and Analysis Using the scp Package -- Bioinformatics Pipeline for Processing Single Cell Data.
520 _aThis volume explores the latest advancements made in the field of mass spectrometry (MS) based single cell proteomics. The chapters in this book focus on technologies, methods, and biological applications and together cover the entire single cell proteomics analysis workflow, from sample collection and preparation through liquid chromatographic separation and mass spectrometric data acquisition to data analysis and interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Mass Spectrometry Based Single Cell Proteomics: Methods and Protocols is a valuable resource for all researchers interested in using these technologies and methods in their laboratories to continue making biological discoveries in this important field.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMass spectroscopy.
650 0 _aSpectrum analysis.
650 0 _aImaging systems in biology.
650 1 4 _aMass Spectrometry.
650 2 4 _aSpectroscopy.
650 2 4 _aBiological Imaging.
700 1 _aVegvari, Akos.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aTeppo, Jaakko.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aZubarev, Roman A.
_eeditor.
_0(orcid)0000-0001-9839-2089
_1https://orcid.org/0000-0001-9839-2089
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071639337
776 0 8 _iPrinted edition:
_z9781071639351
776 0 8 _iPrinted edition:
_z9781071639368
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2817
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3934-4
912 _aZDB-2-PRO
999 _c8301
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