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_a10.1007/978-1-0716-5268-8 _2doi |
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_aSuper-Resolution Microscopy _h[electronic resource] : _bMethods and Protocols / _cedited by Frederic A. Meunier. |
| 250 | _a1st ed. 2026. | ||
| 264 | 1 |
_aNew York, NY : _bSpringer US : _bImprint: Humana, _c2026. |
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_aX, 330 p. 77 illus., 71 illus. in color. _bonline resource. |
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_aMethods in Molecular Biology, _x1940-6029 ; _v3034 |
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| 505 | 0 | _aStructured Illumination Microscopy -- Multicolor super-resolution fluorescence imaging of cells -- Super-resolution spinning-disk confocal microscopy using optical photon reassignment -- Improving STED-based super-resolution-imaging with fluorescence lifetimes -- Single Molecule Localization Microscopy with Fixed Photoactivatable Fluorescent Proteins and Direct Stochastic Optical Reconstruction Microscopy -- Single particle tracking Photo-Activated Localization Microscopy -- Single Molecule Localisation Microscopy in the nucleus with self-labelling tags: Halo, SNAP and CLIP tags -- Single Molecule Localisation Microscopy in vivo at the Drosophila neuromuscular junction -- Tracking synaptic vesicles in live neurons using single-molecule super-resolution microscopy -- Single molecule imaging of endogenous proteins -- Shadow imaging of brain microanatomy in live and fixed tissue -- NanoPlex: A universal strategy to multiplex immunostainings for fluorescence microscopy using secondary nanobodies -- Spatiotemporal analysis of super-resolved live cell molecular trajectory data using nanoscale spatiotemporal indexing clustering -- Case study: Illuminating the nanoscale world of microbiology. | |
| 520 | _aThis volume provides comprehensive methods used to perform super-resolution microscopy and its analysis. Each chapter is written by a leading expert in the field, covering fundamentals of super-resolution microscopy, single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy, expansion microscopy, and others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Super-Resolution Microscopy: Methods and Protocols provides well-established protocols used by both academics and industrials. | ||
| 532 | 8 | _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub. | |
| 532 | 8 | _aNo reading system accessibility options actively disabled | |
| 532 | 8 | _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com | |
| 650 | 0 |
_aMaterials _xMicroscopy. |
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| 650 | 0 | _aImaging systems in biology. | |
| 650 | 1 | 4 | _aMicroscopy. |
| 650 | 2 | 4 | _aBiological Imaging. |
| 700 | 1 |
_aMeunier, Frederic A. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
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_iPrinted edition: _z9781071652671 |
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_iPrinted edition: _z9781071652695 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071652701 |
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_aMethods in Molecular Biology, _x1940-6029 ; _v3034 |
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| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-1-0716-5268-8 |
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