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020 _a9781071651384
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024 7 _a10.1007/978-1-0716-5138-4
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082 0 4 _a571.9642
_223
245 1 0 _aPlant Immunity
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Tsutomu Kawasaki, Hirofumi Yoshioka.
250 _a1st ed. 2026.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2026.
300 _aXI, 266 p. 153 illus., 122 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bPDF/UA-1
_2onix
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v3012
505 0 _aLumi-Map, a Luciferase Bioluminescence Screen of Mutants Combined with MutMap Allows Rapid Dissection of Plant Immune Signaling Pathways -- Identification of Phosphorylation and Protein Interactions of Plant Pattern Recognition Receptors Through Immunoprecipitation -- Plant-Based Ligand-Depletion Binding Assay for Assessing Pattern Recognition Receptor-Ligand Interactions -- Plate Reader-Based Quantification of Cellular Calcium Dynamics in Immune Responses Using the Genetically-Encoded Calcium Indicator GCaMP -- Visualization of Calcium Decoding via a FRET-Based Reporter of Calcium-Decoder Conformation -- Visualization and Quantification of Apoplastic ROS by Plant Immune NADPH Oxidase Utilizing Chemiluminescence -- Detection of Phosphorylated NADPH Oxidase by Antibody During Plant Immune Responses -- The Application of RenSeq for Pan-NLRome Analysis -- Blue Native-PAGE Assay of NLR Protein Complexes Combined with Genetic Complementation in Nicotiana benthamiana -- Quantification of NLR-Induced Cell Death Using Rice Protoplasts -- Split-TurboID-Based Proximity Labeling for Probing the Composition of Spatiotemporally Defined Protein Complexes In Planta -- Virus-Induced Gene Silencing for Identifying Autoimmune Regulators in Arabidopsis -- Bioinformatics Workflow for Co-Transcriptome Analysis of Plant-Bacterial Interactions -- Nuclei Isolation from Mature Leaves of Arabidopsis thaliana Infected by Bacterial Pathogens for Single-Nucleus Multiomics -- Multimodal Analysis of Phytoalexin Synthesis in Arabidopsis by Mass Spectrometry Imaging and Fluorescent Microscopy -- Bioluminescence-Based Growth Quantification of the Phytopathogenic Bacterium Pseudomonas syringae pv. tomato DC3000 -- Genome Editing for Developing Disease-Resistant Plants.
520 _aThis volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, identification of NLR complex components, pan-NLRome analysis, resistosome assays, and cell death evaluation. Additionally, it presents protocols for co-transcriptome analysis of plant-pathogen interactions, single-nucleus multi-omics, metabolite imaging, and bioluminescence-based quantification of pathogen growth, as well as genome editing tools that contribute to the development of disease-resistant plants. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Immunity: Methods and Protocols serves as an ideal guide for researchers aiming to accelerate ongoing research and advances in the field of plant immunity.
532 8 _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub.
532 8 _aNo reading system accessibility options actively disabled
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650 0 _aPlants
_xDisease and pest resistance.
650 0 _aBotany.
650 1 4 _aPlant Immunity.
650 2 4 _aPlant Science.
700 1 _aKawasaki, Tsutomu.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aYoshioka, Hirofumi.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071651377
776 0 8 _iPrinted edition:
_z9781071651391
776 0 8 _iPrinted edition:
_z9781071651407
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v3012
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-5138-4
912 _aZDB-2-PRO
999 _c8263
_d8263