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020 _a9781071626870
_9978-1-0716-2687-0
024 7 _a10.1007/978-1-0716-2687-0
_2doi
050 4 _aQH426-470
072 7 _aPSAK
_2bicssc
072 7 _aSCI029000
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072 7 _aPSAK
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082 0 4 _a576.5
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245 1 0 _aRNA Structure and Dynamics
_h[electronic resource] /
_cedited by Jienyu Ding, Jason R. Stagno, Yun-Xing Wang.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXI, 254 p. 53 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2568
505 0 _aCo-transcriptional Assembly and Native Purification of Large RNA-RNA Complexes for Structural Analyses -- Probing RNA Structures and Interactions using Fluorescence Lifetime Analyses -- Characterizing Fluorescence Properties of Turn-on RNA Aptamers -- Probing Transient Riboswitch Structures via Single Molecule Accessibility Analysis -- Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch using an Interdependent-Sites Binding Model -- Application of Molecular Dynamics to Expand Docking Program’s Exploratory Capabilities and to Evaluate Its Predictions -- Exploring the Binding Process of Cognate Ligand to add Adenine Riboswitch Aptamer by using Explicit Solvent Molecular Dynamics (MD) Simulation -- Prediction of Dynamic RBP-RNA Interactions using PrismNet -- High-resolution Atomic Force Microscopy Imaging of RNA molecules in solution -- 3D Structural Analysis of Long Non-coding RNA by Small Angle X-ray Scattering and Computational Modeling -- Combining Biophysical Methods for Structure-function Analyses of RNA in Solution -- Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA -- Auto-DRRAFTER: Automated RNA Modeling Based on Cryo-EM Density -- X-ray Crystallography to Study Conformational Changes in a TPP Riboswitch -- Preparation of RNA Microcrystals for Serial Femtosecond Crystallography Experiments -- Mix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates.
520 _aThis volume provides a wide spectrum of multidisciplinary approaches for studying RNA structure and dynamics, including detailed accounts of experimental and computational procedures. Chapters guide readers through cryo-electron microscopy, crystallography, isothermal titration calorimetry, small angle X-ray scattering, single-molecule Förster Energy transfer, X-ray free electron laser, atomic force microscopy, computational simulation, and prediction. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, RNA Structure and Dynamics aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aGenetics.
650 0 _aMaterials
_xMicroscopy.
650 1 4 _aGenetics and Genomics.
650 2 4 _aMicroscopy.
700 1 _aDing, Jienyu.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aStagno, Jason R.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWang, Yun-Xing.
_eeditor.
_0(orcid)0000-0002-2175-0148
_1https://orcid.org/0000-0002-2175-0148
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071626863
776 0 8 _iPrinted edition:
_z9781071626887
776 0 8 _iPrinted edition:
_z9781071626894
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2568
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2687-0
912 _aZDB-2-PRO
999 _c8217
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