000			05006nam a22005055i 4500
001			978-3-642-01147-4
003			DE-He213
005			20260610083716.0
007			cr nn 008mamaa
008			100308s2010 gw | s |||| 0|eng d
020			_a9783642011474 _9978-3-642-01147-4
024	7		_a10.1007/978-3-642-01147-4 _2doi
050		4	_aQR180-189.5
072		7	_aMJCM _2bicssc
072		7	_aMED044000 _2bisacsh
072		7	_aMJCM _2thema
082	0	4	_a571.96 _223
082	0	4	_a616.079 _223
245	1	0	_aAntibody Engineering Volume 2 _h[electronic resource] / _cedited by Roland E. Kontermann, Stefan Dübel.
250			_a2nd ed. 2010.
264		1	_aBerlin, Heidelberg : _bSpringer Berlin Heidelberg : _bImprint: Springer, _c2010.
300			_aXII, 589 p. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aSpringer Protocols Handbooks, _x1949-2456
505	0		_aBioinformatics of Antigen-binding Sites -- Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool -- Standardized Sequence and Structure Analysis of Antibody Using IMGT® -- Protein Sequence and Structure Analysis of Antibody Variable Domains -- Generation of Antibody Fragments and Their Derivatives -- scFv by Two-Step Cloning -- Bivalent Diabodies -- Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies) -- Miniantibodies -- Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins -- Antibody–Cytokine Fusion Proteins with Members of the TNF-Family -- Recombinant Immunotoxins for Treating Cancer -- T Bodies -- Expressing Intrabodies in Mammalian Cells -- Phenotypic Knockdown with Intrabodies -- Disulfide-Stabilized Fv Fragments -- PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics -- Fusion Proteins with Improved PK -- In Vivo Biotinylated scFv Fragments -- Bispecific Diabodies and Single-Chain Diabodies -- Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-Ig™) Molecule -- Isolation of Antigen-Specific Nanobodies -- CDR-FR Peptides -- Production of Antibody Fragments -- Purification and Characterization of His-Tagged Antibody Fragments -- Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium -- Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L -- Purification and Analysis of Strep-tagged Antibody-Fragments -- Production of Antibodies and Antibody Fragments in Escherichia coli -- Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones -- Bioreactor Production of scFv Fragments in Pichia pastoris -- Expression of Antibody Fragments in Transgenic Plants -- Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells -- Recombinant Antibody Molecules in Nanobiotechnology and Proteomics -- Immunoliposomes -- Targeted Polymeric Nanoparticles.-Antibody Microarrays for Expression Analysis -- Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique -- Preclinical and Clinical Development -- Xenograft Mouse Models for Tumour Targeting -- Xenograft Mouse Models for Tumour Targeting -- Imaging Tumor Xenografts Using Radiolabeled Antibodies -- Human Anti-antibody Response -- IP Issues in the Therapeutic Antibody Industry.
520			_aAntibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. Antibody Engineering provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.
650		0	_aImmunology.
650		0	_aImmunospecificity.
650	1	4	_aImmunology.
650	2	4	_aAdaptive Immunity.
700	1		_aKontermann, Roland E. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDübel, Stefan. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt
710	2		_aSpringerLink (Online service)
773	0		_tSpringer Nature eBook
776	0	8	_iPrinted edition: _z9783642011467
776	0	8	_iPrinted edition: _z9783642011481
776	0	8	_iPrinted edition: _z9783642422768
830		0	_aSpringer Protocols Handbooks, _x1949-2456
856	4	0	_uhttps://doi.org/10.1007/978-3-642-01147-4
912			_aZDB-2-PRO
999			_c8183 _d8183