000 04505nam a22004575i 4500
001 978-1-59259-285-2
003 DE-He213
005 20260610083715.0
007 cr nn 008mamaa
008 100301s2002 xxu| s |||| 0|eng d
020 _a9781592592852
_9978-1-59259-285-2
024 7 _a10.1385/1592592856
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aCombinatorial Library
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Lisa B. English.
250 _a1st ed. 2002.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2002.
300 _aXI, 383 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v201
505 0 _aLibrary Synthesis and Quality Control -- Using a Noncovalent Protection Strategy to Enhance Solid-Phase Synthesis -- Quality Control of Solid-Phase Synthesis by Mass Spectrometry -- Preparation of Encoded Combinatorial Libraries for Drug Discovery -- Simple Tools for Manual Parallel Solid Phase Synthesis -- Cleavage of Compounds from Solid Phase by Gaseous Reagents -- Synthesis of DNA-Binding Polyamides -- The Preparation of Phenyl-stilbene Derivatives Using the Safety Catch Linker -- Host—Guest Chemistry -- Automated Structure Verification of Small Molecules Libraries Using 1D and 2D NMR Techniques -- Rapid Liquid-Phase Combinatorial Synthesis of Heterocyclic Libraries -- Soluble Polymer-Supported Methods for Combinatorial and Organic Synthesis -- Analytical Methods for Optimization and Quality Control of Combinatorial Synthesis -- Library Purification and Screening -- Resolving Racemic Mixtures Using Parallel Combinatorial Libraries -- Ligand Libraries for the Extraction of Metal Ions -- Automated Liquid-Liquid Extraction and Ion-Exchange Solid-Phase Extraction for Initial Purification -- On-Bead and Solution Screening Approaches for Genomically Derived Targets -- Computational Library Design -- Design of Virtual Combinatorial Libraries -- Approaches to Library Design for Combinatorial Chemistry -- Reagent-Based and Product-Based Computational Strategies in Library Design -- Designing Combinatorial Libraries for Efficient Screening -- Application of Neural Networks to Large Dataset QSAR, Virtual Screening, and Library Design.
520 _aBy significantly increasing the number of targets available for drug discovery, the Human Genome and Proteome projects have made the use of combinatorial libraries essential to developing and optimizing drug candidate molecules more rapidly. Lisa English and a panel of expert researchers have collected in Combinatorial Library Methods and Protocols a novel series of computational and laboratory methods for the design, synthesis, quality control, screening, and purification of combinatorial libraries. Here the reader will find cutting-edge techniques for the preparation of encoded combinatorial libraries, for the synthesis of DNA-binding polyamides, and for combinatorial receptors. There are also state-of-the-art methods for computational library design, quality control by mass spectrometry, and structure verification using 1D and 2D NMR. A variety of well-known computational approaches are provided to meet the information management challenge of multiple biological assays. Each readily reproducible technique includes detailed step-by-step instructions and helpful notes on troubleshooting and avoiding pitfalls. Timely and highly practical, Combinatorial Library Methods and Protocols makes available for all drug discovery researchers all the powerful combinatorial chemistry tools that are increasing the number of candidate compounds and speeding the process of drug discovery and development today.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aEnglish, Lisa B.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896039803
776 0 8 _iPrinted edition:
_z9781489938985
776 0 8 _iPrinted edition:
_z9781489938992
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v201
856 4 0 _uhttps://doi.org/10.1385/1592592856
912 _aZDB-2-PRO
999 _c8128
_d8128