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020 _a9781493963614
_9978-1-4939-6361-4
024 7 _a10.1007/978-1-4939-6361-4
_2doi
050 4 _aQR180-189.5
072 7 _aMJCM
_2bicssc
072 7 _aMED044000
_2bisacsh
072 7 _aMJCM
_2thema
082 0 4 _a571.96
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082 0 4 _a616.079
_223
245 1 0 _aClostridium difficile
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Adam P. Roberts, Peter Mullany.
250 _a2nd ed. 2016.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2016.
300 _aXI, 292 p. 49 illus., 33 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1476
505 0 _aRestriction Endonuclease Analysis Typing of Clostridium Difficile Isolates -- Direct PCR-ribotyping of Clostridium difficile -- From FASTQ to Function: In silico Methods for Processing Next Generation Sequencing Data -- Clostridium difficile Genome Editing Using pyrE Alleles -- Use of mCherryOpt Fluorescent Protein in Clostridium difficile -- A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile -- Clostridium difficile Adhesins -- Intestinal Epithelial Cell Response to Clostridium difficile Flagella -- Isolating and Purifying Clostridium difficile Spores -- Inducing and Quantifying Clostridium difficile Spore Formation -- Characterization of Functional Prophages in Clostridium difficile -- Induction and Purification of C. difficile Phage Tail-like Particles -- Phage Transduction -- Transfer of Clostridium difficile Genetic Elements Conferring Resistance to Macrolide-Lincosamide-streptograminB (MLSB) Antibiotics -- Methods for Determining Transfer of Mobile Genetic Elements in Clostridium difficile -- Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus between Clostridium difficile Strains -- An in vitro Model of the Human Colon: Studies of Intestinal Biofilms and lostridium difficile Infection -- MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community -- A Practical Method for Preparation of Fecal Microbiota Transplantation -- Ion Exchange Chromatography to Analyze Components of a Clostridium difficile Vaccine -- A Size Exclusion Chromatography Method for Analysis of Clostridium difficile Vaccine Toxins.
520 _aThis second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis which are used to investigate the fundamental biology of the organism and its virulence factors. Additional chapters describe development of potential new treatments including vaccines, bacteriophage and faecal transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols, Second Edition provides a comprehensivecatalogue of molecular tools and techniques authored by the researchers who have developed them.
650 0 _aImmunology.
650 1 4 _aImmunology.
700 1 _aRoberts, Adam P.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMullany, Peter.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493963591
776 0 8 _iPrinted edition:
_z9781493963607
776 0 8 _iPrinted edition:
_z9781493981762
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1476
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-6361-4
912 _aZDB-2-PRO
999 _c8125
_d8125