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001 978-1-59259-027-8
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020 _a9781592590278
_9978-1-59259-027-8
024 7 _a10.1007/978-1-59259-027-8
_2doi
050 4 _aTP248.13-248.65
072 7 _aTCB
_2bicssc
072 7 _aSCI010000
_2bisacsh
072 7 _aTCB
_2thema
082 0 4 _a660.6
_223
245 1 0 _aDownstream Processing of Proteins
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Mohamed A. Desai.
250 _a1st ed. 2000.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2000.
300 _aIX, 229 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Biotechnology,
_x1940-607X ;
_v9
505 0 _aDownstream Processing in the Biotechnology Industry -- 2 Microbial Cell Disruption by High-Pressure Homogenization -- 3 Protein Concentration and Buffer Exchange Using Ultrafiltration -- 4 High-Performance Tangential Flow Filtration for Protein Separations -- Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation -- Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules -- Application of Chromatography in the Downstream Processing of Biomolecules -- Affinity Ligand Technology -- Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules -- Expanded Bed Adsorption in the Purification of Biomolecules -- Application of HPLC in the Purification of Biomolecules -- Countercurrent Chromatography of Proteins with Polymer Phase Systems -- Practical Aspects and Applications of Radial Flow Chromatography -- Sterile Filtration and Virus Filtration -- Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products -- Validation of Sterilizing Filters in the Biotechnology Industry.
520 _aIn Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods with a potential for scale-up. Also treated are the various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology. Every bioprocess scientist and engineer working to design and validate biological processes for novel proteins-and successfully apply for their new product licenses-will find this important book an eminently practical resource.
650 0 _aBiotechnology.
650 1 4 _aBiotechnology.
700 1 _aDesai, Mohamed A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896035645
776 0 8 _iPrinted edition:
_z9781617370755
776 0 8 _iPrinted edition:
_z9781489941794
830 0 _aMethods in Biotechnology,
_x1940-607X ;
_v9
856 4 0 _uhttps://doi.org/10.1007/978-1-59259-027-8
912 _aZDB-2-PRO
999 _c8122
_d8122