000 04657nam a22005175i 4500
001 978-1-60761-700-6
003 DE-He213
005 20260610083715.0
007 cr nn 008mamaa
008 100427s2010 xxu| s |||| 0|eng d
020 _a9781607617006
_9978-1-60761-700-6
024 7 _a10.1007/978-1-60761-700-6
_2doi
050 4 _aQP34-38
072 7 _aMFG
_2bicssc
072 7 _aSCI036000
_2bisacsh
072 7 _aMFG
_2thema
082 0 4 _a612
_223
245 1 0 _aMembrane Transporters in Drug Discovery and Development
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Qing Yan.
250 _a1st ed. 2010.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2010.
300 _aXIII, 357 p. 60 illus., 2 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v637
505 0 _aMembrane Transporters and Drug Development: Relevance to Pharmacogenomics, Nutrigenomics, Epigenetics, and Systems Biology -- Bioinformatics for Transporter Pharmacogenomics and Systems Biology: Data Integration and Modeling with UML -- Multidrug Resistance: Phylogenetic Characterization of Superfamilies of Secondary Carriers that Include Drug Exporters -- Targeting Drug Transporters – Combining In Silico and In Vitro Approaches to Predict In Vivo -- Methods to Evaluate Transporter Activity in Cancer -- Analysis of Expression of Drug Resistance-Linked ABC Transporters in Cancer Cells by Quantitative RT-PCR -- Fluorescence Studies of Drug Binding and Translocation by Membrane Transporters -- A Model for Transport Studies of the Blood–Brain Barrier -- Genetic Variants in the Vesicular Monoamine Transporter 1 (VMAT1/SLC18A1) and Neuropsychiatric Disorders -- Equilibrium Binding and Transport by Vesicular Acetylcholine Transporter -- ABC Transporters in Ophthalmic Disease -- Imaging of Protein Translocation In Situ in Skeletal Muscle of Living Mice -- Glucose Transporters in Parasitic Protozoa -- NMR Studies of Membrane Proteins -- Site-Directed Mutagenesis in the Study of Membrane Transporters -- Xenopus laevis Oocytes -- Measurement of Intracellular pH -- Measurement of Plasma Membrane Calcium–Calmodulin-Dependent ATPase (PMCA) Activity -- Assessment of the Contribution of the Plasma Membrane Calcium ATPase, PMCA, Calcium Transporter to Synapse Function Using Patch Clamp Electrophysiology and Fast Calcium Imaging.
520 _aStudies of membrane transporters have had a great impact on our understanding of human diseases and the design of effective drugs. In Membrane Transporters in Drug Discovery and Development: Methods and Protocols, expert researchers provide practical methodologies of the ongoing research on membrane transporters, considering applications of transporter technologies in drug discovery and development. Chapters include new and useful fields and methodologies, including pharmacogenomics, nutrigenomics, systems biology, bioinformatics, nuclear magnetic resonance (NMR), imaging, and quantitative real-time-PCR. Transporter studies in drug discovery and development for various diseases are discussed, including neuropsychiatric disorders, cardiovascular diseases, ophthalmic diseases, cancer, and diabetes. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and current, Membrane Transporters in Drug Discovery and Development: Methods and Protocols delivers a collection of practical protocols that can be used immediately in the lab, along with critical surveys of key topics by leading researchers in the field.
650 0 _aHuman physiology.
650 0 _aPharmacology.
650 0 _aBiological transport.
650 0 _aCell membranes.
650 1 4 _aHuman Physiology.
650 2 4 _aPharmacology.
650 2 4 _aMembrane Trafficking.
700 1 _aYan, Qing.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781607616993
776 0 8 _iPrinted edition:
_z9781607617150
776 0 8 _iPrinted edition:
_z9781493957330
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v637
856 4 0 _uhttps://doi.org/10.1007/978-1-60761-700-6
912 _aZDB-2-PRO
999 _c8115
_d8115