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020 _a9781597453639
_9978-1-59745-363-9
024 7 _a10.1007/978-1-59745-363-9
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aG-Quadruplex DNA
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Peter Baumann.
250 _a1st ed. 2010.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2010.
300 _aXII, 276 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v608
505 0 _aG-Quadruplexes: From Guanine Gels to Chemotherapeutics -- Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes -- Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential -- Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis -- Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay -- Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy -- Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures -- 2-Aminopurine as a Probe for Quadruplex Loop Structures -- Assessing DNA Structures with 125I Radioprobing -- Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques -- Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments -- Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC -- Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography -- Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes -- G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands.
520 _aRecent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In G-Quadruplex DNA: Methods and Protocols, experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, G-Quadruplex DNA: Methods and Protocols promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.
650 0 _aBiochemistry.
650 0 _aCytology.
650 0 _aMedical genetics.
650 1 4 _aBiochemistry.
650 2 4 _aCell Biology.
650 2 4 _aMedical Genetics.
700 1 _aBaumann, Peter.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588299505
776 0 8 _iPrinted edition:
_z9781607614920
776 0 8 _iPrinted edition:
_z9781493956395
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v608
856 4 0 _uhttps://doi.org/10.1007/978-1-59745-363-9
912 _aZDB-2-PRO
999 _c8112
_d8112