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020 _a9781592592388
_9978-1-59259-238-8
024 7 _a10.1385/1592592384
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aGene Probes
_h[electronic resource] :
_bPrinciples and Protocols /
_cedited by Marilena Aquino do Muro, Ralph Rapley.
250 _a1st ed. 2002.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2002.
300 _aXI, 272 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v179
505 0 _aTarget Format and Hybridization Conditions -- End Labeling Procedures -- Photobiotin Labeling -- Nick Translation -- Random Primed Labeling -- Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization -- Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors -- The ?-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites -- Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma -- Detection of Chlamydia trachomatis by DNA Amplification -- Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes -- Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction -- Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes -- Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4 -- Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes) -- Analysis of 16S–23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species -- Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes -- In Situ Hybridization of Burkholderia cepacia Cells -- Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil -- Detection of Single-Copy Genes in DNA from Transgenic Plants -- Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission -- Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food -- Polymerase Chain Reaction for Detection of Listeria monocytogenes.
520 _aThe use of nucleic acid or gene probes as cloning or diagnostic tools has promoted significant advances in human genome mapping and in the isolation and manipulation of genes generally. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using gene probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Each cutting-edge method includes practical hints and tips and is described in step-by-step detail by an expert who not only routinely uses the technique, but has also optimized it for experimental success. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions. Comprehensive and versatile, Gene Probes: Principles and Protocols provides a cutting-edge practical resource for all biochemists, microbiologists, and molecular biologists who are using this powerful new technology to advance medical, environmental, and food research today.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aAquino do Muro, Marilena.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRapley, Ralph.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896038851
776 0 8 _iPrinted edition:
_z9781617372360
776 0 8 _iPrinted edition:
_z9781489938978
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v179
856 4 0 _uhttps://doi.org/10.1385/1592592384
912 _aZDB-2-PRO
999 _c8095
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