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020 _a9781071637180
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024 7 _a10.1007/978-1-0716-3718-0
_2doi
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_2bicssc
072 7 _aSCI007000
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072 7 _aPSE
_2thema
082 0 4 _a570.724
_223
245 1 0 _aMammalian Synthetic Systems
_h[electronic resource] /
_cedited by Francesca Ceroni, Karen Polizzi.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXI, 316 p. 88 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2774
505 0 _aA directed evolution protocol for engineering minimal transcription factors, based on CIS display -- Set-up and applications of Modular Protein Expression Toolboxes (MoPET) for mammalian systems -- Coiled-coiled interaction toolbox for engineering mammalian cells -- A mammalian-based synthetic biology toolbox to engineer membrane-membrane interfaces -- Engineered protease-responsive RNA Binding Proteins (RBPs) to expand the toolbox of synthetic circuits in mammalian cells -- Mechanistic model-driven biodesign in mammalian synthetic biology -- Realizing Antithetic Integral Feedback Control in Mammalian Cells -- A computational modeling approach for the design of genetic control systems that respond to transcriptional activity -- Flux Balance Analysis of Mammalian Cell Systems -- Multiplex functional characterization of protein variant libraries in mammalian cells with single-copy genomic integration and high throughput DNA sequencing -- Flow Cytometry Quantification of Transient Transfections in Mammalian CellsRNA switches using Cas proteins -- Multiplexed transactivation of mammalian cells using dFnCas12a-VPR -- Anti-CRISPR Proteins and their Application to Control CRISPR-effectors in Mammalian Systems -- Post-translational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells -- Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells -- Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells -- High-throughput spectroscopic analysis of mRNA capping level -- In vitro generation of megakaryocytes from engineered mouse embryonic stem cells -- Preparation of chromobodies for the detection of cell surface epitopes.
520 _aThis volume details cutting-edge methods that enables engineering of novel functions in mammalian cells. Chapters guide readers through cell-to-cell communication, cell fate control, protein and RNA-based biosensors together with tools for more reliable and faster mammalian genome editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Synthetic Systems aims to ensure successful results in the further study of this vital field.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aSynthetic Biology.
650 0 _aBiological models.
650 1 4 _aSynthetic Biology.
650 2 4 _aBiological Models.
700 1 _aCeroni, Francesca.
_eeditor.
_0(orcid)0000-0001-7237-4982
_1https://orcid.org/0000-0001-7237-4982
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aPolizzi, Karen.
_eeditor.
_0(orcid)0000-0001-5435-2667
_1https://orcid.org/0000-0001-5435-2667
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071637173
776 0 8 _iPrinted edition:
_z9781071637197
776 0 8 _iPrinted edition:
_z9781071637203
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2774
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3718-0
912 _aZDB-2-PRO
999 _c8048
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