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020 _a9781071636251
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024 7 _a10.1007/978-1-0716-3625-1
_2doi
050 4 _aQH491-492
072 7 _aPSC
_2bicssc
072 7 _aSCI072000
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072 7 _aPSC
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082 0 4 _a571.8
_223
245 1 0 _aTeratogenicity Testing
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Luís Félix.
250 _a2nd ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXVI, 627 p. 113 illus., 96 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2753
505 0 _aUpdating an Overview of Teratology -- Guidelines on Development Toxicity Tests: Brief Insights -- Development Features on the Selection of Animal Models for Teratogenic Testing -- Virus as Teratogenic Agents -- The Future of the Teratogenicity Testing -- The First Steps on AOPs' Concepts, Development, and Applications in Teratology -- Machine Learning to Predict Teratogenicity: Theory and Practice -- Human Pluripotent Stem Cell-Based Assays to Predict Developmental Toxicity -- Developmental Toxicity Using the Rat Whole Embryo Culture -- A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs -- Teratogenic Effects of Drugs on Primary Lymphocytes Assessed by Flow Cytometry -- Assessment of the Teratogenic Effect of Drugs on the Chicken Embryo -- Daphnia magna as a Model Organism to Predict the Teratogenic Effect of Different Compounds -- Caenorhabditis elegans as an In Vivo Model Organism to Elucidate Teratogenic Effects -- Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans after Developmental Lead Exposure -- Exploration of Teratogenic and Genotoxic Effects on Model Organism Drosophila melanogaster -- Ecotoxicological Assessment of Seaweed-Based Crop Biostimulant on Earthworm Eudrilus eugeniae Kinb -- Evaluation of the Ecotoxicology of Seaweed-Based Biopesticide Used in Combat of the Polyphagous Pest Using Eudrilus eugeniae Kinb -- Biochemical Studies to Understand Teratogenicity and Lethality Outcomes in Modified-FETAX -- Bioinformatics Methods for Transcriptome Analysis on Teratogenesis Testing -- Angiogenesis Assay for Live and Fixed Zebrafish Larvae -- High-Resolution Respirometry for the Assessment of Teratogenic Chemicals -- A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae -- Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos -- Assessment of Developmental Neurotoxicity Using Semi-Automatic Behavior Analysis System for Zebrafish -- Behavioral Profiling of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle Response -- Alcian Blue Staining for Chondrocranium Development in Zebrafish -- Protocol of Geometric Morphometrics for Teratogenicity Testing -- Biochemical Markers for Liver Injury in Zebrafish Larvae -- Cortisol Quantification for Assessing Stress-Induced Changes in Zebrafish Larvae -- Metabolomic Fingerprint Assay in Zebrafish Embryos -- Alkaline Comet Assay to Assess Genotoxicity in Zebrafish Larvae -- A Chromogenic Quantification of Protein Expression in Zebrafish Larvae -- Confocal Microscopy Technique in Teratogenicity Testing Using Zebrafish (Danio rerio) Embryos as Model -- Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos -- Quantitative Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos -- Microarray Analysis to Determine Gene Expression Changes in Zebrafish Embryos -- Cell-Free DNA and Next-GenerationSequencing for Prenatal Diagnosis.
520 _aThis fully updated volume collects laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. Beginning with a section detailing basic principles of teratogenicity, the book continues with application of in vitro models for teratogenic screening to predict toxicity in embryonic animals and humans, as well as a variety of well-established in vivo animal model tests, which offer solutions for evaluating the efficacy and the teratogenicity of compounds in preclinical trials before human safety testing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Teratogenicity Testing: Methods and Protocols, Second Edition serves as an ideal guide to tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aDevelopmental biology.
650 0 _aToxicology.
650 0 _aEmbryology.
650 1 4 _aDevelopmental Biology and Stem Cells.
650 2 4 _aMedical Toxicology.
650 2 4 _aEmbryology.
700 1 _aFélix, Luís.
_eeditor.
_0(orcid)0000-0001-9870-6666
_1https://orcid.org/0000-0001-9870-6666
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071636244
776 0 8 _iPrinted edition:
_z9781071636268
776 0 8 _iPrinted edition:
_z9781071636275
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2753
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3625-1
912 _aZDB-2-PRO
999 _c8040
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