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020 _a9781071635117
_9978-1-0716-3511-7
024 7 _a10.1007/978-1-0716-3511-7
_2doi
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072 7 _aPST
_2bicssc
072 7 _aSCI011000
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072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aPlant Peptide Hormones and Growth Factors
_h[electronic resource] /
_cedited by Andreas Schaller.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXI, 314 p. 59 illus., 46 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2731
505 0 _aBioinformatics Methods for Prediction of Gene Families Encoding Extracellular Peptides -- Identification of Bioactive Phytocytokines Using Transcriptomic Data and Plant Bioassays -- Purification of Phytaspases Using a Biotinylated Peptide Inhibitor -- Characterization of Phytaspase Proteolytic Activity Using Fluorogenic Peptide Substrates -- Characterization of prolyl-4 hydroxylase substrate specificity using Pichia pastoris as an effective eukaryotic expression system -- Extraction of Apoplastic Peptides for the Structural Elucidation of Mature Peptide Hormones in Arabidopsis -- Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings -- A Seedling Growth Inhibition Assay to Measure Phytocytokine Activity -- A Trojan Horse Aproach Using Ustilago maydis to Study Apoplastic Maize (Zea mays) Peptides in situ -- Feeding Assay to Study the Effect of Phytocytokines on Direct and Indirect Defense in Maize -- A Quick Method to Analyze Peptide-Regulated Anthocyanin Biosynthesis -- Quantitative Measurement of Pattern-Triggered ROS Burst as an Early Immune Response in Tomato -- Automated Real-Time Monitoring of Extracellular pH to Assess Early Plant Defense Signaling -- Peptide-Mediated Cyclic Nucleotide Signaling in Plants: Identification and Characterization of Interactor Proteins with Nucleotide Cyclase Activity -- Detection of Ligand-Induced Receptor Kinase and Signaling Component Phosphorylation With Mn2+-Phos-Tag SDS-PAGE -- In-vivo Cross Linking of Biotinylated Peptide Ligands to Cell Surface Receptors -- Evaluation of Direct Ligand-Receptor Interactions by Photoaffinity Labeling -- Rapid Identification of Peptide-Receptor-Coreceptor Complexes in Protoplasts -- Acridinium-Based Chemiluminescent Receptor-Ligand Binding Assay for Protein/Peptide Hormones -- LuBiA (Luciferase-based Binding Assay): Glowing Peptides as Sensitive Probes to Study Ligand-Receptor Interactions -- Microscale Thermophoresis (MST) to Study Rapid Alkalinization Factor (RALF) - Receptor Interactions -- Isothermal Titration Calorimetry to Study Plant Peptide Ligand-Receptor Interactions. .
520 _aThis book describes state-of-the-art approaches for identification and functional characterization of plant peptide hormones and growth factors. Chapters guide readers through protocols for computational identification of novel signaling peptides and phytocytokines, analysis of post-translational maturation events, isolation of mature peptides from plant tissues, peptide bioassays, in vivo and in vitro analysis of peptide activity, peptide receptor identification and interaction analysis, and quantitative assessment of peptide ligand binding to cognate receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Peptide Hormones and Growth Factors aims to be a useful and practical guide to new researchers and experts looking to expand their technical know-how.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBotany.
650 1 4 _aPlant Science.
700 1 _aSchaller, Andreas.
_eeditor.
_0(orcid)0000-0001-6872-9576
_1https://orcid.org/0000-0001-6872-9576
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071635100
776 0 8 _iPrinted edition:
_z9781071635124
776 0 8 _iPrinted edition:
_z9781071635131
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2731
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3511-7
912 _aZDB-2-PRO
999 _c8028
_d8028