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024 7 _a10.1007/978-1-0716-3495-0
_2doi
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072 7 _aSCI017000
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082 0 4 _a571.6
_223
245 1 0 _aTrophoblasts
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Sandeep Raha.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXII, 248 p. 50 illus., 46 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2728
505 0 _aIsolation and Maintenance in Culture of Primary Human Trophoblast from term Placentae -- Isolation of Human Villous Cytotrophoblastic Cells from Term Placenta for Transfection -- Enzymatic Digestion and Single Cell Isolation of Peri-Implantation Stage Human Trophoblast Cells -- Precision-Cut Slice Culture Method for Rat Placenta -- Evaluation of Molecular Interactions and Cellular Dynamics at the Maternal-Fetal Interface during Placental Morphogenesis -- In Vivo Quantitative Assessment of Gestational Choriocarcinoma Development and Progression using Luminescent Trophoblast Cells -- Analyzing Trophoblast Fusion using Immunofluorescence and Split Protein Complementation Assays -- Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling -- Assessment of Placental Sodium Independent Leucine Uptake and Transfer in Trophoblast Cells -- Assessing Cholesterol Efflux on Primary Human Trophoblast Cells -- RGD-Based Fluorescenceto Assess Placental Angiogenesis -- Real-Time Assessment of Mitochondrial Function in Cytotrophoblast and Syncytialized Trophoblast Cells using the Seahorse XFe24 Extracellular Flux Analyzer -- Quantifying Trophoblast Function using the xCELLigence System -- Liposome Encapsulated Anti-Inflammatory Proteins for targeted Delivery to the Placenta to Treat Fetal Growth Restriction -- Trophoblast-Targeted Lipsomes for Placenta-Specific Drug Delivery -- Methods for Co-Culture of Primary Human Extravillous Trophoblast Cells and Uterine Natural Killer Cells -- Trophoblast Organoids as a Novel Tool to Study Human Placental Development and Function -- A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening -- Three-Dimensional In Vitro Human Placental Organoids from Mononuclear Villous Trophoblasts or Trophoblast Stem Cells to Understand Trophoblast Dysfunction in Fetal Growth Restriction.
520 _aThis volume explores the latest approaches used to assess trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion. The chapters in this book cover topics such as various methods to study and manipulate primary trophoblast cells; strategies using liposomes to deliver biomolecules to trophoblasts; in vitro models of the placenta that emulate the cellular interactions in the 3D uterine environment; and models exploring heterogenous cell types and 3D-organoid structures that represent cell-to-cell interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Trophoblasts: Methods and Protocols is a valuable resource that will help researchersbetter understand the function of the placenta and its complex contribution to maternal health and fetal development, and also provide greater insights to the area of study known as the developmental origins of health and diseases (DoHaD). .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCytology.
650 0 _aEmbryology.
650 1 4 _aCell Biology.
650 2 4 _aEmbryology.
700 1 _aRaha, Sandeep.
_eeditor.
_0(orcid)0000-0001-7307-3292
_1https://orcid.org/0000-0001-7307-3292
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071634943
776 0 8 _iPrinted edition:
_z9781071634967
776 0 8 _iPrinted edition:
_z9781071634974
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2728
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3495-0
912 _aZDB-2-PRO
999 _c8027
_d8027