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020 _a9781071635490
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024 7 _a10.1007/978-1-0716-3549-0
_2doi
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_2bicssc
072 7 _aSCI045000
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245 1 0 _aBacteriophages
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Ebenezer Tumban.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXVI, 431 p. 47 illus., 36 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2738
505 0 _aStructural and genomic diversity of bacteriophages -- The diversity of bacteriophages in human gut -- Breaking the ice - A review of phages from polar ecosystems -- The diversity of bacteriophages in hot spring -- Isolation of bacteriophage from soil samples in a poorly equipped field lab in Kruger National Park -- Purification and up-concentration of bacteriophages and viruses from fecal samples -- Isolation of enterococcus phages from municipal wastewater samples using an enrichment step -- Phage DNA extraction, genome assembly, and genome closure -- Enumeration of bacteriophages by plaque assay -- Detection and quantification of phages in wastewater samples by culture and molecular methods -- Flow virometry: A fluorescence-based approach to enumerate bacteriophages in liquid samples -- A metagenomics approach to enumerate bacteriophages in a Food Niche -- Bioinformatic analysis of Staphylococcus phages: a key step for safe cocktail development -- Use of localized reconstruction to visualize the Shigella-bacteriophage Sf6 tail apparatus -- Bacteriophage-host interactions and co-evolution -- Unraveling physical interactions of Clostridioides difficile with phage and phage-derived proteins using in vitro and whole-cell assays -- Phage Transduction of Staphylococcus aureus -- The Next Generation of Drug Delivery: Harnessing the Power of Bacteriophages -- Construction of nonnatural cysteine-crosslinked phage libraries -- Application of next generation sequencing in phage display -- The application of bacteriophage and photoacoustic flow cytometry in bacterial identification -- Propagation, purification and characterization of bacteriophages for phage -- Overcoming bacteriophage resistance in phage therapy -- .Bacteriophage virus-like particles: platforms for vaccine design.
520 _aThis volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small to jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture, molecular techniques, protocols for isolate, interaction of bacteriophage proteins with host cells, and how to use bacteriophages to transfer foreign genetic elements to bacterial strains. In addition to the above, chapters feature the application of bacteriophages/bacteriophage-derived products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips (in the Notes section) on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacteriophages: Methods and Protocols aims to ensure successful results in further study of this vital field.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMicrobiology.
650 0 _aBacteria.
650 0 _aImmunology.
650 1 4 _aMicrobiology.
650 2 4 _aBacteria.
650 2 4 _aImmunology.
700 1 _aTumban, Ebenezer.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071635483
776 0 8 _iPrinted edition:
_z9781071635506
776 0 8 _iPrinted edition:
_z9781071635513
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2738
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3549-0
912 _aZDB-2-PRO
999 _c8026
_d8026