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020 _a9781071634776
_9978-1-0716-3477-6
024 7 _a10.1007/978-1-0716-3477-6
_2doi
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072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aXylem
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Javier Agusti.
250 _a2nd ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXI, 245 p. 50 illus., 45 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2722
505 0 _aMonitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes -- Modeling and analyzing xylem vulnerability to embolism as an epidemic process -- Modeling Xylem Functionality Aspects -- Detecting and quantifying xylem embolism by synchrotron-based x-ray micro-CT -- Analysis of xylem cells by nucleus-based transcriptomics and chromatin profiling -- Quantification of xylem-specific thermospermine-dependent translation of SACL transcripts with dual luciferase reporter system -- Fossil wood analyses: several examples from five case studies in the area of Central and NW Bohemia, Czech Republic -- Isolation and reproductive structures induction of fungal pathogens associated with xylem and wood necrosis in grapevine -- Determination of de novo suberin-lignin ferulate deposition in xylem tissue upon vascular pathogen attack -- Quantification of treacheary elements types in mature hypocotyl of Arabidopsis thaliana -- Histochemical Detection of Peroxidase and Laccase Activities in Populus Secondary Xylem -- Lignin analysis by HPLC and FTIR: spectra deconvolution and S/G ratio determination -- Inducible pluripotent suspension cell cultures (iPSCs) to study plant cell differentiation -- Bulk and in situ quantification of coniferaldehyde residues in lignin -- Clearing of vascular tissue in Arabidopsis thaliana for reporter analysis of gene expression.
520 _aThis second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Xylem: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBotany.
650 1 4 _aPlant Science.
700 1 _aAgusti, Javier.
_eeditor.
_0(orcid)0000-0002-6459-2928
_1https://orcid.org/0000-0002-6459-2928
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071634769
776 0 8 _iPrinted edition:
_z9781071634783
776 0 8 _iPrinted edition:
_z9781071634790
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2722
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3477-6
912 _aZDB-2-PRO
999 _c8025
_d8025