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020 _a9781071634295
_9978-1-0716-3429-5
024 7 _a10.1007/978-1-0716-3429-5
_2doi
050 4 _aR850.A1-854
050 4 _aQH315-320
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072 7 _aMED106000
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245 1 0 _aVascular Hyperpermeability
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Binu Tharakan.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aX, 264 p. 69 illus., 54 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2711
505 0 _aDetermination of Solute Permeability of Microvascular Endothelial Cell Monolayers In Vitro -- Evaluation of Vascular Permeability in Inflamed Vessels of the Cremaster Muscle in Live Mice -- Lymphatic Vascular Permeability Determined from Direct Measurements of Solute Flux -- Evaluation of Mesenteric Microvascular Hyperpermeability Following Hemorrhagic Shock using Intravital Microscopy -- Determination of Endothelial Barrier Resistance by Electric Cell-Substrate Impedance Sensing (ECIS) System -- Time-Lapse Observation of Cell Dynamics during Angiogenesis Using the Rat Mesentery Culture Model -- Evaluation of Barrier Integrity using a Two-Layered Microfluidic Device Mimicking the Blood-Brain Barrier -- Intravital Imaging of Leukocyte-Endothelial Interaction in Hindlimb Ischemia/Reperfusion Injury by Intravital Multiphoton Microscopy -- Studying Angiogenesis using Matrigel In Vitro and In Vivo -- Determination of Blood-Brain Barrier Hyperpermeability Using Intravital Microscopy -- Imaging and Analysis of the Dynamics of Filamentous Actin Structures in Live Endothelial Cells -- Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs) -- Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification -- Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay -- Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model -- Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells -- An In Vitro Bilayer Model of Human Primary Retinal Pigment Epithelial and Choroid Endothelial Cells for Permeability Studies -- Quantifying Adhesion of Inflammatory Cells to the Endothelium In Vitro -- Determination of Tight Junction Integrity in Brain Endothelial Cells Based on Tight Junction Protein Expression -- Evaluation of Glycolysis and Mitochondrial Function in Endothelial Cells Using the Seahorse Analyzer -- Evaluation of Tight Junction Integrity in Brain Endothelial Cells using Confocal Microscopy.
520 _aThis volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human umbilical vein endothelial cells; measurement of blood-brain barrier hyperpermeability using Evans Blue extravasation assay; lymphatic vascular permeability determined from direct measurements of solute flux; intravital imaging of leukocyte-endothelial interaction by intravital multiphoton microscopy; and evaluation of tight junction integrity in brain endothelial cells using confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Vascular Hyperpermeability: Methods and Protocols is a valuable resource that will aid novice researchers with creating new and affordable research projects, and for expert researchers to initiate new strategies and collaborations in their ongoing programs. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMedicine
_xResearch.
650 0 _aBiology
_xResearch.
650 0 _aInternal medicine.
650 1 4 _aBiomedical Research.
650 2 4 _aInternal Medicine.
700 1 _aTharakan, Binu.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071634288
776 0 8 _iPrinted edition:
_z9781071634301
776 0 8 _iPrinted edition:
_z9781071634318
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2711
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3429-5
912 _aZDB-2-PRO
999 _c8023
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