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020 _a9781071626511
_9978-1-0716-2651-1
024 7 _a10.1007/978-1-0716-2651-1
_2doi
050 4 _aR850.A1-854
050 4 _aQH315-320
072 7 _aMBGR
_2bicssc
072 7 _aMED106000
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072 7 _aMBGR
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082 0 4 _a610.72
_223
245 1 0 _aRetinitis Pigmentosa
_h[electronic resource] /
_cedited by Stephen H. Tsang, Peter M.J. Quinn.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXVII, 404 p. 100 illus., 40 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2560
505 0 _aIntroduction and History of Retinitis Pigmentosa -- Etiology of Retinitis Pigmentosa -- Clinical Evaluation of Patients with Retinitis Pigmentosa -- Molecular Genetic Testing Approaches for Retinitis Pigmentosa -- Pedigree Analysis of Families and Patients Affected by Retinitis Pigmentosa -- Whole Genome Sequencing for Detection of Structural Variants in Patients with Retinitis Pigmentosa -- Fundus Imaging Methodologies to Assess RP patients -- The Use of Optical Coherence Tomography in Evaluation of Retinitis Pigmentosa -- Optical Coherence Tomography Angiography (OCTA) findings in Retinitis Pigmentosa -- ERG to Evaluate the Retina in Cases of Retinitis Pigmentosa -- Visual Function Tests: Visual Fields -- Visual Function Tests -- Visual Acuity as a Measurement of Visual Function -- Measure of Visual Function -- Ophthalmic Fluorescein Angiography -- Indocyanine Green Angiography -- Use of the Medmont Dark-Adapted Chromatic Perimeter for Assessing Rod Function in Retinitis Pigmentosa -- Multi-Luminance Mobility Testing Endpoint -- Preclinical Models of Retinitis Pigmentosa -- ERG to evaluate the retina using mouse models -- Autofluorescence Imaging to Evaluate Retinal Disease Progression in Rodent -- Large animal models of retinitis pigmentosa in therapy development and preclinical testing -- CRISPR manipulations in stem cell lines -- CRISPR-mediated genome engineering in cell lines -- CRISPR off-targeting -- CRISPR manipulations in stem cell lines -- Generation of Human iPSC-Derived Retinal Organoids for Assessment of AAV-Mediated Gene Delivery -- Culture of Human Retinal Explants for Ex Vivo Assessment of AAV Gene Delivery -- Prime Editing for the Installation and Correction of Mutations causing Inherited Retinal Disease: A Brief Methodology -- Large scale single-nucleus RNA sequencing compatible with complex archived samples -- Vector Shedding and Immunogenicity Sampling in AAV-based Gene Therapy for Retinitis Pigmentosa Patients -- Current Management Options for Patients with Retinitis Pigmentosa -- Challenges of Treatment Methodologies and the Future of Gene Therapy and Stem Cell Therapy to Treat Retinitis Pigmentosa -- Ocular Injection Techniques for Retinitis Pigmentosa: Intravitreal, Subretinal, and Suprachoroidal -- Surgical Approach with Pars Plana Vitrectomy for Subretinal Gene Therapy.
520 _aThis volume details the history of Retinitis Pigmentosa and current treatment options. Chapters guide readers through CRISPR, gene therapy, stem cell therapy, next-generation sequencing methods, gene editing, and translational applications of other therapies to the treatment of Retinitis Pigmentosa. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinitis Pigmentosa aims to be a useful practical guide to researches to help further their study in this field. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMedicine
_xResearch.
650 0 _aBiology
_xResearch.
650 0 _aOphthalmology.
650 1 4 _aBiomedical Research.
650 2 4 _aOphthalmology.
700 1 _aTsang, Stephen H.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aQuinn, Peter M.J.
_eeditor.
_0(orcid)0000-0001-9940-4264
_1https://orcid.org/0000-0001-9940-4264
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071626504
776 0 8 _iPrinted edition:
_z9781071626528
776 0 8 _iPrinted edition:
_z9781071626535
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2560
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2651-1
912 _aZDB-2-PRO
999 _c8021
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