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020 _a9781603272926
_9978-1-60327-292-6
024 7 _a10.1007/978-1-60327-292-6
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aMouse Molecular Embryology
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Mark Lewandoski.
250 _a1st ed. 2014.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2014.
300 _aXIV, 465 p. 107 illus., 71 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1092
505 0 _aIn situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase Staining -- Two Color In Situ Hybridization of Whole-Mount Mouse Embryos -- Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections -- Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays -- EMAGE: Electronic Mouse Atlas of Gene Expression -- Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue -- Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach -- Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression -- Generation of Tissue Organoids by Compaction Reaggregation -- Ultra-Rapid Vitrification of Mouse Oocytes and Embryos -- Mammalian Preimplantation Embryo Culture -- Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs -- Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia -- Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms -- Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos -- Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues -- Protein/Peptide Transduction in Metanephric Explant Culture -- Direction of Cells Programmed to Die in Mouse Embroys -- Microscopic Computed Tomography-based  Virtual Histology of Embryos -- Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study -- The Latest Improvements in the Mouse Sperm Preservation -- Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro -- Using the Textpresso SiteSpecific Recombinases Web Server to Identify  Cre Expressing Mouse Strains and Floxed Alleles -- Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing -- Genetic Cell Ablation -- Essentials of Recombinase-based Genetic Fate Mapping in Mice.
520 _aIn Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study  mouse embryology.  These include protocols and techniques that  are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA.  With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.    Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.
650 0 _aCytology.
650 0 _aEmbryology.
650 1 4 _aCell Biology.
650 2 4 _aEmbryology.
700 1 _aLewandoski, Mark.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781603272902
776 0 8 _iPrinted edition:
_z9781461487838
776 0 8 _iPrinted edition:
_z9781489977731
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1092
856 4 0 _uhttps://doi.org/10.1007/978-1-60327-292-6
912 _aZDB-2-PRO
999 _c8016
_d8016