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020 _a9781592592111
_9978-1-59259-211-1
024 7 _a10.1385/1592592112
_2doi
050 4 _aRB155-155.8
072 7 _aMFN
_2bicssc
072 7 _aMED107000
_2bisacsh
072 7 _aMFN
_2thema
082 0 4 _a616.042
_223
245 1 0 _aGenomic Imprinting
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Andrew Ward.
250 _a1st ed. 2002.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2002.
300 _aXII, 377 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v181
505 0 _aGeneration of Monoparental Embryos for Investigation into Genomic Imprinting -- Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting -- Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome -- Production of YAC Transgenic Mice by Pronuclear Injection -- A Transgenic Approach to Studying Imprinted Genes -- Methylation-Sensitive Genome Scanning -- Subtraction-Hybridization Method for the Identification of Imprinted Genes -- Identification of Imprinted Loci by Methylation -- Ribonuclease Protection -- Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression -- Allele-Specific In Situ Hybridization (ASISH) -- RNA-FISH to Analyze Allele-Specific Expression -- Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes -- Southern Analysis Using Methyl-Sensitive Restriction Enzymes -- A PCR-Based Method for Studying DNA Methylation -- Bisulfite-Based Methylation Analysis of Imprinted Genes -- Direct Analysis of Chromosome Methylation -- In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs -- In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation -- Detection of Methyl?Sensitive DNA?Binding Proteins with Possible Involvement in the Imprinting Phenomenon -- Probing Chromatin Structure with Nuclease Sensitivity Assays -- Examining Histone Acetlylation at Specific Genomic Regions -- Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis -- Reconstitution of Chromatin In Vitro -- Genomic Imprinting in Plants.
520 _aImprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning experiments. In Genomic Imprinting: Methods and Protocols, Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. The majority of protocols describe molecular techniques that allow examination of gene structure or expression in an allele-specific manner. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC, and YAC constructs. Each technique is described in step-by-step detail to ensure successful results. Incorporating a wealth of knowledge from leading exponents in the field, Genomic Imprinting: Methods and Protocols brings together all the essential molecular, genetic, and embryological methods commonly used in today's laboratories for the identification and analysis of imprinted genes.
650 0 _aMedical genetics.
650 1 4 _aMedical Genetics.
700 1 _aWard, Andrew.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896037410
776 0 8 _iPrinted edition:
_z9781617371646
776 0 8 _iPrinted edition:
_z9781489938954
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v181
856 4 0 _uhttps://doi.org/10.1385/1592592112
912 _aZDB-2-PRO
999 _c7995
_d7995