000 04650nam a22005055i 4500
001 978-1-4939-1695-5
003 DE-He213
005 20260610083712.0
007 cr nn 008mamaa
008 141009s2015 xxu| s |||| 0|eng d
020 _a9781493916955
_9978-1-4939-1695-5
024 7 _a10.1007/978-1-4939-1695-5
_2doi
050 4 _aQK
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aAgrobacterium Protocols
_h[electronic resource] :
_bVolume 1 /
_cedited by Kan Wang.
250 _a3rd ed. 2015.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Springer,
_c2015.
300 _aXIV, 368 p. 42 illus., 29 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1223
505 0 _aGateway® Compatible Plant Transformation Vectors.- Brachypodium Distachyon -- Medicago Truncatula Transformation Using Cotyledonary Explants -- Medicago Truncatula Transformation Using Leaf Explants -- Setaria Viridis -- Barley (Hordeum Vulgare L.) Transformation Using Immature Embryos -- Barley (Hordeum Vulgare L.) Transformation Using Embryogenic Pollen Cultures -- Maize (Zea Mays L.) -- Maize, Tropical (Zea Mays L.). - Finger Millet [Eleusine Coracana (L.) Gaertn] -- Oat (Avena Sativa L.) -- Rice, Indica (Oryza Sativa L.) -- Rice, Japonica (Oryza Sativa L.) -- Sorghum (Sorghum Bicolor L.) -- Wheat (Triticum Aestivum L.) Transformation Using Immature Embryos -- Wheat (Triticum Aestivum L.) Transformation Using Mature Embryos -- Alfalfa (Medicago Sativa L.) -- Clovers (Trifolium Spp.) -- Clover, Red (Trifolium Pratense) -- Cowpea [Vigna Unguiculata (L.) Walp] -- Lentil (Lens Culinaris Medik.) -- Soybean [Glycine Max (L.) Merr.] -- Brassica Oleracea And B. Napus -- Cucumber (Cucumis Sativus L.) and Kabocha Squash (Cucurbita Moschata Duch) -- Pepper, Chili (Capsicum Annuum) -- Pepper, Chili (Capsicum Annuum) -- Pepper, Sweet (Capsicum Annuum) -- Sugar Beet (Beta Vulgaris L.) -- Tomato (Solanum Lycopersicum).
520 _aRapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.   Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and  three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis.  It also updates a chapter for vector construction, a step critical to a successful plant transformation process.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition  facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.
650 0 _aBotany.
650 0 _aPlant genetics.
650 0 _aBiotechnology.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
650 2 4 _aChemical Bioengineering.
700 1 _aWang, Kan.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493916948
776 0 8 _iPrinted edition:
_z9781493916962
776 0 8 _iPrinted edition:
_z9781493945412
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1223
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-1695-5
912 _aZDB-2-PRO
999 _c7964
_d7964