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020 _a9781592596799
_9978-1-59259-679-9
024 7 _a10.1385/1592596797
_2doi
050 4 _aRC1-1245
072 7 _aMJC
_2bicssc
072 7 _aMED022000
_2bisacsh
072 7 _aMJC
_2thema
082 0 4 _a616
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245 1 0 _aMolecular Diagnosis of Infectious Diseases
_h[electronic resource] /
_cedited by Jochen Decker, Udo Reischl.
250 _a2nd ed. 2004.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2004.
300 _aXVI, 464 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v94
505 0 _aProtein-Based Identification Of Antigens -- Proteomic Approaches to Antigen Discovery -- Immunoproteomics -- Immunoprecipitation and Blotting -- DNA-Based Identification of Antigens -- Representational Difference Analysis of cDNA -- Microarray Data Analysis and Mining -- Expression Cloning -- Identification of Epitopes and Immunomodulatory Components -- Determination of Epitopes by Mass Spectrometry -- Identification of T-Cell Epitopes Using ELISpot and Peptide Pool Arrays -- Virus-like Particles -- Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components -- Expression of Recombinant Proteins -- High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format -- Production of Antigens in Chlamydomonas reinhardtii -- Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA -- Purification, Modification, and Renaturation of Recombinant Proteins -- Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) -- Purification of Recombinant Proteins with High Isoelectric Points -- Refolding of Inclusion Body Proteins -- Small-Molecule-Protein Conjugation Procedures -- Characterization of Recombinant Proteins -- Structural Characterization of Proteins and Peptides -- Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors -- Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor -- Evaluation of Recombinant Proteins in Immunological Test Systems -- Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays -- Design and Preparationof Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays -- Basic Problems of Serological Laboratory Diagnosis -- Molecular Diagnostics Resources on the Internet -- Recombinant Receptor Molecules -- Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells.
520 _aIn this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references. Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.
650 0 _aDiseases.
650 1 4 _aDiseases.
700 1 _aDecker, Jochen.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aReischl, Udo.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588292216
776 0 8 _iPrinted edition:
_z9781617374302
776 0 8 _iPrinted edition:
_z9781489939548
830 0 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v94
856 4 0 _uhttps://doi.org/10.1385/1592596797
912 _aZDB-2-PRO
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_d7933