000 04622nam a22005055i 4500
001 978-1-59259-562-4
003 DE-He213
005 20260610083711.0
007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592595624
_9978-1-59259-562-4
024 7 _a10.1385/0896033554
_2doi
050 4 _aQD71-142
072 7 _aPNF
_2bicssc
072 7 _aSCI013010
_2bisacsh
072 7 _aPNF
_2thema
082 0 4 _a543
_223
245 1 0 _aGlycoanalysis Protocols
_h[electronic resource] /
_cedited by Elizabeth F. Hounsell.
250 _a2nd ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aX, 262 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v76
505 0 _aCharacterization of Protein Glycosylation -- Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides -- Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography -- Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM) -- HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column -- HPLC and HPAEC of Oligosaccharides and Glycopeptides -- Analysis of N- and O-Glycans by Pyridylamination -- Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins -- Analysis of Glycosaminoglycans and Proteoglycans -- The Use of Cell and Organ Culture for the Study of Secreted Mucins -- Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides -- TLC in Structure and Recognition Studies of Glycosphingolipids -- Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids -- Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinos¡tol Structures -- Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique.
520 _aNow fully updated and considerably expanded, Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working with today's pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd ed., continues with vital, time-tested techniques addressing the needs of both biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or described non-text content (images, graphs etc). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at accessibilitysupport@springernature.com if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aAnalytical chemistry.
650 1 4 _aAnalytical Chemistry.
700 1 _aHounsell, Elizabeth F.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896033559
776 0 8 _iPrinted edition:
_z9781617370168
776 0 8 _iPrinted edition:
_z9781489942616
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v76
856 4 0 _uhttps://doi.org/10.1385/0896033554
912 _aZDB-2-PRO
999 _c7914
_d7914