000 03824nam a22004575i 4500
001 978-1-59259-722-2
003 DE-He213
005 20260610083711.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592597222
_9978-1-59259-722-2
024 7 _a10.1385/159259722X
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aConfocal Microscopy
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Stephen W. Paddock.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _aXII, 446 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v122
505 0 _aAn Introduction to Confocal Imaging -- Practical Considerations for Collecting Confocal Images -- Fluorescent Probes for Confocal Microscopy -- Imaging Gene Expression Using Antibody Probes -- Single and Double FISH Protocols for Drosophila -- Confocal Microscopy of Plant Cells -- Preparation of Yeast Cells for Confocal Microscopy -- Confocal Methods for Caenorhabditis elegans -- Imaging Sea Urchin Fertilization -- Imaging Immunolabeled Drosophila Embryos by Confocal Microscopy -- Confocal Microscopy on Xenopus laevis Oocytes and Embryos -- Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos -- Confocal Imaging of Living Cells in Intact Embryos -- Live Confocal Analysis with Fluorescently Labeled Proteins -- Live Imaging with Green Fluorescent Protein -- Fluorescent Calcium Indicators:Subcellular Behavior and Use in Confocal Imaging -- Intracellular pH and pCa Measurement -- Measuring Dynamic Cell Volume In Situ by Confocal Microscopy -- Imaging Thick Tissues with Confocal Microscopy -- Measurement in the Confocal Microscope -- Presentation of Confocal Images -- The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides -- Information Management of Confocal Microscopy Images -- Morphing Confocal Images and Digital Movie Production.
520 _aIn Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aPaddock, Stephen W.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896035263
776 0 8 _iPrinted edition:
_z9781617370625
776 0 8 _iPrinted edition:
_z9781489943392
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v122
856 4 0 _uhttps://doi.org/10.1385/159259722X
912 _aZDB-2-PRO
999 _c7886
_d7886