| 000 | 05885nam a22005175i 4500 | ||
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| 001 | 978-1-59259-597-6 | ||
| 003 | DE-He213 | ||
| 005 | 20260610083710.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100301s1998 xxu| s |||| 0|eng d | ||
| 020 |
_a9781592595976 _9978-1-59259-597-6 |
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| 024 | 7 |
_a10.1385/0896034852 _2doi |
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| 050 | 4 | _aRC1-1245 | |
| 072 | 7 |
_aMJC _2bicssc |
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| 072 | 7 |
_aMED022000 _2bisacsh |
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| 072 | 7 |
_aMJC _2thema |
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| 082 | 0 | 4 |
_a616 _223 |
| 245 | 1 | 0 |
_aMolecular Diagnosis of Infectious Diseases _h[electronic resource] / _cedited by Udo Reischl. |
| 250 | _a1st ed. 1998. | ||
| 264 | 1 |
_aTotowa, NJ : _bHumana Press : _bImprint: Humana, _c1998. |
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| 300 |
_aXV, 629 p. 126 illus. _bonline resource. |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
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| 490 | 1 |
_aMethods in Molecular Medicine, _x1940-6037 ; _v13 |
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| 505 | 0 | _aRecombinant Proteins and Peptide -- Identification of Novel Diagnostic Markers by Differential Display -- Immunoprecipitation -- RT-PCR Methods and Applications -- Quantitative RT-PCR -- Cloning cDNA Ends Using RACE -- A Method for the Rapid Identification of Epitopes and Other Functional Peptide Domains -- Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis -- Amplification of Genomic DNA by PCR -- PCR-Based Cloning and Subsequent Expression of Antigenic Proteins in Escherichia coli -- Application of TEV Protease in Protein Production -- Stable Transfected Drosophila Schneider-2 Cells as a Novel Tool to Produce Recombinant Antigens for Diagnostic, Therapeutic, and Preventive Purposes -- Use of Recombinant Antigens as Diagnostic Reagents in Enzyme-Linked Immunosorbent Assays -- Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector -- The SFV Gene Expression System -- Expression and Purification of Recombinant Proteins Using the pET System -- Procedures for the Analysis and Purification of His-Tagged Proteins -- Detection and Immobilization of Proteins Containing the 6xHis Tag -- The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins -- Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) -- Purification of Recombinant Proteins Having High Isoelectric Points -- Immunoblotting -- Diagnostic Application of a Multiple-Channel Immunoblot Apparatus -- Immobilization of lmmunoreactants on Solid Phases for ELlSAs -- Nonradioactive Labeling and lmmunodetection of Recombinant Proteins -- Flow Cytometry -- Basic Problems of Serological Laboratory Diagnosis -- Molecular Diagnostics Resources on the Internet -- Antibodies -- Monoclonal Antibody-Based Immunoassays -- Biosensor-Based Methods in Clinical Diagnosis -- The Quartz Crystal Microbalance (QCM) as an Immunosensor -- Biomolecular Interaction Analysis -- Recent Developments in Antibody Engineering -- Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas -- Production of a Human Antibody Library in the Phage-Display Vector pSEX81 -- Screening of Phage-Displayed Antibody Libraries -- Purification, Characterization, and Biotinylation of Single-Chain Antibodies. | |
| 520 | _aIn Molecular Diagnosis of Infectious Diseases leading international authorities provide a comprehensive and up-to-date set of basic and advanced methods that illuminate the principles of molecular diagnostics and allow researchers to establish assay systems tailored to their specific needs. This unique collection of protocols covers the latest strategies for identifying novel diagnostic markers, epitope mapping, and the production and purification of recombinant antigens, as well as their use as diagnostic reagents in immunological assays. It also includes promising techniques based on recent developments in antibody engineering and on the use of biosensors. Molecular Diagnosis of Infectious Diseases offers clinical microbiologists, clinical chemists, and pathologists unique tools with which to develop improved assays for diagnosing infectious diseases. The book is certain to play a leading role in helping researchers to understand and realize in their daily work the powerful potential of molecular diagnostic techniques in clinical microbiology today. | ||
| 532 | 8 | _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or descriptions for non-text content (e.g., images and graphs). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at through this accessibility request webform if you require assistance or an alternative format. | |
| 532 | 8 | _aInaccessible, or known limited accessibility | |
| 532 | 8 | _aNo reading system accessibility options actively disabled | |
| 532 | 8 | _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com | |
| 650 | 0 | _aDiseases. | |
| 650 | 1 | 4 | _aDiseases. |
| 700 | 1 |
_aReischl, Udo. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9780896033986 |
| 776 | 0 | 8 |
_iPrinted edition: _z9780896034853 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489943002 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489943019 |
| 830 | 0 |
_aMethods in Molecular Medicine, _x1940-6037 ; _v13 |
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| 856 | 4 | 0 | _uhttps://doi.org/10.1385/0896034852 |
| 912 | _aZDB-2-PRO | ||
| 999 |
_c7848 _d7848 |
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